Phytochemical analysis of Allium ampeloprasum for the identification of secondary metabolites with osteoprotective properties

Postgraduate Thesis uoadl:1327412 310 Read counter

Unit:
Κατεύθυνση Φαρμακογνωσία βιοδραστικών φυσικών προϊόντων
Library of the School of Science
Deposit date:
2017-01-31
Year:
2016
Author:
NAKA IOANNA
Supervisors info:
Αναπληρωτής Καθηγητής Αληγιάννης Νεκτάριος
Original Title:
Μελέτη του είδους Allium ampeloprasum για την απομόνωση δευτερογενών μεταβολιτών με πιθανή δράση στην οστεοπόρωση
Languages:
Greek
Translated title:
Phytochemical analysis of Allium ampeloprasum for the identification of secondary metabolites with osteoprotective properties
Summary:
The purpose of this thesis was the phytochemical investigation of ethyl acetate and methanolic extract of Allium αmpeloprasum in order to discover natural products related to the prevention and treatmentofosteoporosis. Taking into consideration relevant information of traditional medicine based on texts of Dioscorides, the biological activity of Allium ampeloprasum extracts concerning their estrogenic and osteoblastic activity, as well as their chemical profile these extracts were selected for further analysis.
After the plant collection the ethyl acetate and methanolic extracts were obtained and their chemical profile was investigated using HPLC and HPTLC techmiques. Furthermore the above mentioned extracts were fractionated by use of centrifugal partition chromatography (CPC), and the fractions were further submitted to several chromatographic techniques (such as: column chromatography with solid phase sephadex LH-20 or silica gel and preparative TLC). All the isolated compounds were identified using with the use of nuclear magnetic resonance spectroscopy (NMR) and GC-MS.
In total 23 metabolites were identified belonging to the categories of phenolic acids, flavonoids, sapogenins, saponins, lignans and dihydrobenzofurans. The majority of the isolated compounds havepreviously been reported in Allium species, whereas three metabolites (ΑΑΜ5, ΑΑΜ6, ΑΑΜ9) are reported in Allium ampeloprasum for the first time and two compounds (ΑΑΜ10 and ΑΑΜ13) are identified for the firsttime as natural compounds.
To conclude, between our future goals is the completion of the phytochemical analysis of the extracts Alliumαmpeloprasum and also the biological evaluation of the isolated compounds concerning their estrogenic and osteoprotective activity.
Main subject category:
Science
Other subject categories:
Pharmacy
Keywords:
Allium ampeloprasum, osteoporosis, phytoestrogens, saponins, lignans, flavonoids, phenolic compounds, phytochemical analysis
Index:
Yes
Number of index pages:
2
Contains images:
Yes
Number of references:
103
Number of pages:
187
Νάκα Ιωάννα Μεταπτυχιακή εργασία.pdf (6 MB) Open in new window