Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότηταςLibrary of the School of Science
Supervisors info:
Ε. ΠΑΝΤΕΡΗ, Αναπληρώτρια Καθηγήτρια ΕΚΠΑ, Φαρμακευτική Σχολή
Ι. ΛΟΥΚΑΣ, Αναπληρώτης Καθηγητής ΕΚΠΑ, Φαρμακευτική Σχολή
Ι. ΝΤΟΤΣΙΚΑΣ, Επίκουρος Καθηγητής ΕΚΠΑ, Φαρμακευτική Σχολή
Original Title:
ΑΝΑΠΤΥΞΗ ΚΑΙ ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΟΥ UPLC/QTOF-MS ΓΙΑ ΤΗΝ ΑΝΙΧΝΕΥΣΗ ΤΩΝ ΠΕΠΤΙΔΙΩΝ ΑΠΕΛΕΥΘΕΡΩΣΗΣ ΤΗΣ ΑΥΞΗΤΙΚΗΣ ΟΡΜΟΝΗΣ (GHRP) ΣΕ ΟΥΡΑ ΑΘΛΗΤΩΝ
Translated title:
Development and validation of a UPLC/QTOF-MS method for the detection of growth hormone releasing peptidew (GHRPs) in athletes' urine
Summary:
The purpose of this Master Thesis was the development and the validation of a UPLC/QTOF-MS method for the detection of growth hormone releasing peptides in the urine of athletes. Specifically, the peptides that were studied are the following: Desmopressin, TB500, Alexamorelin, GHRP2, GHRP4, GHRP5, GHRP6 2-5, GHRP6 2-6, GHRP6 2-6 FREE ACID, LHRH 2-10, Narfarelin, Narfarelin 5-10 Ipamorelin, TB500 1-5 and TB500 1-3.
For the sample processing ion-exchange cartridges were utilized. The filler material has a nonpolar end and a caboxyl group.
The analysis of the samples was performed by Liquid Chromatography-High Performance System (UPLC) combined with Mass Spectrometer Detector and the use of a Time of Flight mass analyzer coupled with a quadrupole (LC / QTOF-MS) analyzer. Analytes where ionized with an electrospray ionization, performed in the positive mode [(+)ESI].
For the detection of the peptides it was necessary to achieve a minimization of the matrix effect phenomenon so as to obtain chromatograms with obvious and clear peaks of monoionized and deionized compouds. Various modifications were tested by changing the column, the experimental procedure and in the spectrometric parameters until there the perfect conditions for the detection of growth hormone releasing peptides were reached.
For the evaluation and validation of the method, the relative retention time was calculated along with the specificity of the analytical method for each detected substance, the ability to identify prohibited substances, the precision of the method, carry-over effect and the matrix effect.
Overall, the method was considered as adequate and suitable for the intended purpose.
Main subject category:
Science
Other subject categories:
Pharmacy
Keywords:
peptides
UPLC/QTOF-MS
MS
validation
development
growth hormone realeasing factors
GHRP
athletes
urine
File:
File access is restricted only to the intranet of UoA.
ΤΕΛΙΚΟ-MASTER-ΞΙΟΥΡΗ ΜΑΡΙΑ 2017.pdf
4 MB
File access is restricted only to the intranet of UoA.
