Synthesis of diblock terpolymer poly(ethylene oxide)-b-poly(L-histidine-co-glycine) and study of the effect of L-glycine on the aggregation and the secondary structure

Postgraduate Thesis uoadl:1663070 423 Read counter

Unit:
Κατεύθυνση Επιστήμη Πολυμερών και Εφαρμογές της
Library of the School of Science
Deposit date:
2017-06-10
Year:
2017
Author:
Santorinaios Konstantinos
Supervisors info:
Ερμόλαος Ιατρού, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Σύνθεση δισυσταδικών τριπολυμερών πολύ(αιθυλενοξειδίου)-b-πολύ(L-ιστιδίνη-co-γλυκίνη) και μελέτη της επίδρασης της L-γλυκίνης στη συσσωμάτωση και τη δευτεροταγή δομή
Languages:
Greek
Translated title:
Synthesis of diblock terpolymer poly(ethylene oxide)-b-poly(L-histidine-co-glycine) and study of the effect of L-glycine on the aggregation and the secondary structure
Summary:
The purpose of this thesis is the synthesis of diblocks containing L-Histidine, that possess the ability to respond to external stimuli by altering their structure. It is presented a novel method for the synthesis of Nim-Trt-L-Histidine NCA with Trityl protecting group, which gives the advantages of selective and easy deprotection and no racemization. Also the synthesis of glycine NCA took place. The successful synthesis of the monomers was confirmed with IR and NMR techniques. The successful synthesis of co-diblocks with addition of PGly in rates of 10%, 20% and 40% in relation with histidine in the second block of the polymers took place beginning with m-PEO-NH2 as macroinitiator in the first block. The ROP method for the polymerization of the NCAs of the aminoacids took place in combination with the use of high vacuum techniques. All polymers were characterized with GPC, NMR and IR techniques. The polymers have the ability of self-assembly by shaping in nanoscale micelles and clusters in water as solvent. This ability was confirmed with DLS. These polypeptides can respond in pH change. A study of the structure of the polymers took place by using circular dichroism. The different molds of the polymer were found by changing pH and temperature.
Main subject category:
Science
Other subject categories:
Chemistry
Keywords:
polypeptides, NCAs, self-assembly, bioapplications
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
87
Number of pages:
148
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