Prevalence of herpesviruses HSV-1 and HSV-2 in subgingival plaque and their association with chronic periodontitis

Postgraduate Thesis uoadl:1702966 580 Read counter

Unit:
Κατεύθυνση Βιολογία Στόματος (Βασικές Οδοντιατρικές Επιστήμες)
Βιβλιοθήκη Οδοντιατρικής
Deposit date:
2017-07-05
Year:
2017
Author:
Chatzopoulou Eirini
Supervisors info:
Δερέκα Ξανθίππη, Επίκουρη Καθηγήτρια Περιοδοντολογίας, Οδοντιατρική Σχολή ΕΚΠΑ
Κιτράκη Ευθυμία, Καθηγήτρια Βιολογίας, Εργαστήριο Βασικών Ιατροβιολογικών Επιστημών, Οδοντιατρική Σχολή ΕΚΠΑ
Μπομπέτσης Γεώργιος, Επίκουρος Καθηγητής Περιοδοντολογίας, Οδοντιατρική Σχολή ΕΚΠΑ
Original Title:
Μελέτη ανίχνευσης των ερπητοϊών HSV-1 και HSV-2 και ο πιθανός ρόλος τους στην παθογένεια της περιοδοντικής νόσου
Languages:
Greek
Translated title:
Prevalence of herpesviruses HSV-1 and HSV-2 in subgingival plaque and their association with chronic periodontitis
Summary:
Prevalence of herpesviruses HSV-1 and HSV-2 in subgingival plaque and their association with chronic periodontitis.
Background
Several studies have investigated the occurrence of herpes simplex virus (HSV) in chronic periodontitis. However, a possible association of HSV with periodontal disease is not yet established, while limited information is available on the prevalence of types HSV-1 and HSV-2 in chronic periodontitis patients.
Aim
The aim of the present study was a) to compare the prevalence of HSV-1 and HSV-2 in subgingival plaque samples from patients with severe chronic periodontitis to periodontally healthy individuals and b) to determine a possible association between viral detection and clinical parameters.
Materials and methods
Subgingival plaque samples from 26 chronic periodontitis patients (CP group) and 11 individuals with healthy periodontium (control group) were evaluated. In the control group, clinical parameters were recorded and one pooled sample of subgingival plaque was collected from each control subject. In the CP group, periodontal clinical indices were assessed and subgingival plaque samples were collected. Then, the CP group received non-surgical periodontal therapy. After 4 to 6 weeks, clinical parameters were recorded and subgingival plaque samples were collected from the same sites as at baseline. Each CP patient contributed with three subgingival plaque samples: one pooled sample from the mesial sites of the 1st molars, one from the deepest pocket in the dentition and one from a shallow site. A nested PCR was employed to determine the presence of HSV-1 and HSV-2.



Results
In the CP group, 42.3% and 30.8% of the patients were positive at least in one sample for HSV-1 and HSV-2, respectively. In the control group, the respective percentages were 27.3% and 18.2%. The differences in viral detection between the CP and the control group were not statistically significant for any virus. No difference was found in the detection rate of HSV-1 and HSV-2 among the three sample groups (pooled, deep, shallow) in the CP group. With regard to clinical parameters, no association was observed for the presence of HSV-2, while HSV-1 was inversely correlated with whole mouth plaque index and periodontal pocket depth.
Conclusions
The results of the present study do not support a possible role of HSV-1 and HSV-2 in the pathogenesis of chronic periodontitis in a Greek population. Further studies are recommended in order to delineate the association of these viruses with periodontal disease. The hypothesis that the presence of HSV-1 is correlated with decreased periodontal pocket depth due to immunological dysregulation in periodontal tissues should be tested.
Main subject category:
Health Sciences
Other subject categories:
Dentistry
Keywords:
herpesvirus, HSV-1, HSV-2, chronic periodontitis
Index:
No
Number of index pages:
0
Contains images:
No
Number of references:
170
Number of pages:
91
File:
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