Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Author:
Dimou Argiro-Ioanna
Supervisors info:
Μιχαήλ Κουππάρης, Καθηγητής, Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Ανάπτυξη και επικύρωση μεθόδου HPLC για ποσοτικό προσδιορισμό και έλεγχο προσμίξεων του βαλπροϊκού νατρίου και προσδιορισμό EDTA σε ενέσιμο διάλυμα
Translated title:
Development and validation of the assay and related substances HPLC method of sodium valproate and assay of EDTA in injection solution
Summary:
In this research project three HPLC methods with photodiode array detector for the determination of sodium valproate and its related substances and disodium edetate were developed and validated. Valproic acid is a carboxylic acid and commonly used for the treatment of epilepsy and as a mood stabilizing agent for a variety of psychiatric disorders.
For the sodium valproate and its related substances a BDS Hypersil C18 column with isocratic elution was applied using mobile phase consisting of a mixture of phosphate buffer pH=5,0 and methanol. The flow rate was 1,0 ml/min and the quantification wavelength was set at 210 nm. For the disodium edetate a Spherisorb ODS2 column was used, with isocratic elution using a mobile phase consisting of acetonitrile and tetrabutylammonium hydroxide solution with pH= 6,5, flow rate at 2,0 mL/min and the quantification wavelength was set at 254 nm.
For the validation of all the developed methods analytical characteristics, such as linearity, accuracy, specifity, precision and robustness were investigated, along with detection and quantification limits.
Main subject category:
Science
Other subject categories:
Analytical chemistry
Keywords:
sodium valproate, disodium edetate, impurities, HPLC, method validation
