Study of the expression levels of Fibrillin-1 (FBN1) in oral squamous cell carcinomas

Postgraduate Thesis uoadl:1708715 491 Read counter

Κατεύθυνση Βιολογία Στόματος (Βασικές Οδοντιατρικές Επιστήμες)
Βιβλιοθήκη Οδοντιατρικής
Deposit date:
Gkatzogianni Margarita
Supervisors info:
Κιτράκη Ευθυμία, Καθηγήτρια Βιολογίας Οδοντιατρικής Σχολής ΕΚΠΑ
Τόσιος Κωνσταντίνος, Επίκουρος Καθηγητής Παθολογίας και Χειρουργικής Στόματος, Εργαστήριο Στοματολογίας Οδοντιατρικής Σχολής ΕΚΠΑ
Γκιζάνη Σωτηρία, Επίκουρη Καθηγήτρια Παιδοδοντιατρικής, Εργαστήριο Παιδοδοντιατρικής Οδοντιατρικής Σχολής ΕΚΠΑ
Original Title:
Μελέτη της γονιδιακής έκφρασης της Φιμπριλίνης-1 (FBN1) σε ακανθοκυτταρικά καρκινώματα στόματος
Translated title:
Study of the expression levels of Fibrillin-1 (FBN1) in oral squamous cell carcinomas
Fibrillin- 1 (Fbn1) is a large glycoprotein, which is an essential structural component of the extracellular matrix, and its microfibrils provide flexibility to the tissues, but they can also be involved in the signaling pathways of various growth factors. Mutations in the gene encoding Fbn1 can cause diseases of the connective tissue (type 1 fibrillinopathies), while in previous studies changes in gene expression of Fbn1 have been associated with several types of cancer, but not with oral squamous cell carcinoma, which is the most common malignant neoplasm in the head and neck area.
The aim of this study was to investigate the mRNA levels of Fbn1 in oral squamous cell carcinoma. Specifically, 24 biopsy samples from patients with oral squamous cell carcinoma (OSCC) and 8 samples from people with traumatic fibroma (control group) from the archives of the Oral Pathology Department of the Dental School of Athens were used. At first, total RNA was isolated from paraffin sections and cDNA was prepared by reverse transcription. Then, real time polymerase chain reaction (RT- qPCR) was conducted and the results were statistically analyzed, using non parametric methods.
The results showed that the samples from patients with oral squamous cell carcinoma exhibit statistically significant decreased mRNA levels of Fbn1 compared with the control group (traumatic fibromas). The OSCC samples derived from women show a higher expression of Fbn1 compared with those of men, however that difference is not statistically significant. Fbn1 levels appear higher in moderately differentiated tumors, followed by well and poorly differentiated carcinomas, but the differences are not statistically significant. Age also does not affect the expression of Fbn1.
Although there are studies in the literature that correlate changes in the expression of Fbn1 with several types of cancer, the present study is the first one associating Fbn1 with oral squamous cell carcinoma. The observed reduction in mRNA levels of Fbn1 in OSCC samples may indicate a connection between that change and the appearance of the malignancy. Further studies are required in order to understand the mechanism of these changes in the expression of Fbn1 in OSCC and its possible differential expression according to the degree of tumor differentiation.
Main subject category:
Health Sciences
fibrillin-1, cancer, expression, RT- qPCR, mRNA
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