Vitrification of immature human oocytes (GV stage) from stimulated cycles and In vitro maturation: influence on the survival, fertilization, and blastocyst stage

Postgraduate Thesis uoadl:1722702 291 Read counter

Unit:
ΠΜΣ Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
2017-07-17
Year:
2017
Author:
Karagianni Mairi
Supervisors info:
Λουτράδης Δημήτριος, Καθηγητής Μαιευτικής - Γυναικολογίας, Ιατρικής Σχολής, Εθνικό Και Καποδιστριακό Πανεπιστήμιο Αθηνών
Δρακάκης Πέτρος, Αναπληρωτής Καθηγητής Μαιευτικής - Γυναικολογίας, Ιατρικής Σχολής, Εθνικό Και Καποδιστριακό Πανεπιστήμιο Αθηνών
Καλλιανίδης Κωνσταντίνος, Επίκουρος Καθηγητής Μαιευτικής - Γυναικολογίας, Ιατρικής Σχολής, Εθνικό Και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Μελέτη της επίδρασης της υαλοποίησης (VITRIFICATION) ανθρώπινων ανώριμων (GV) ωαρίων από διεγερμένους κύκλους σε συνδυασμό με τη μέθοδο in vitro ωρίμανσης ωαρίων (IVM) στην ανάπτυξη εμβρύων μέχρι το στάδιο της βλαστοκύστης
Languages:
Greek
Translated title:
Vitrification of immature human oocytes (GV stage) from stimulated cycles and In vitro maturation: influence on the survival, fertilization, and blastocyst stage
Summary:
In the last decade, cryopreservation of oocytes started to be gaining further ground with the use of closed system vitrification method which is proven to be particularly effective. Cryopreservation of oocytes is being mostly referred to mature (MII) oocytes, while immature GV oocytes are being discharged because of their non-synchronized maturation. However, it is very interesting the fact that by using the method of in vitro maturation in combination with suitable conditions, the GV oocytes have the potential to reach maturation and be capable of fertilization. In this way, the number of available oocytes would increase and, thus, would the number of competent embryos for embryo transfer (ET).
In this thesis, the effect of vitrification with closed system on the immature GV oocytes and their developmental progress to the blastocyst stage, after their in vitro maturation, has been investigated. For this purpose, rescued immature (GV) oocytes from donor women, who have undergone ovarian stimulation, were examined in relation to whether and how they can survive of the cryopreservation - using the vitrification method – mature, and be exploited in assisted reproduction techniques.
The demonstrated results are comparable and statistically significant among other correlated studies. This specific method could support the increase of the available number of embryos number for couples who undergo the procedure of assisted reproduction with appropriate, future modifications.
Main subject category:
Health Sciences
Keywords:
Immature oocytes, Vitrification Of Oocytes, IVM In Vitro Maturation
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
148
Number of pages:
129
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