Unit:
Κατεύθυνση Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Author:
Koukounia Marilena
Supervisors info:
Γεώργιος Διαλλινάς, Καθηγητής, Τμήμα Βιολογίας, ΕΚΠΑ
Nicholas Skandalis, Associate Professor of Research Medicine, Keck School of Medicine, USC
Αμαλία Καραγκούνη-Κύρτσου, Καθηγήτρια, Τμήμα Βιολογίας, ΕΚΠΑ
Δημήτριος Χατζηνικολάου, Αναπληρωτής Καθηγητής, Τμήμα Βιολογίας, ΕΚΠΑ
Original Title:
Διερεύνηση του ρόλου των σιδηροφορέων στη βιοχειραγώγηση φυτικών συστημάτων
Translated title:
Investigation of the role of siderophores in biomanipulation of plant systems
Summary:
The effect of the commercial strain Bacillus amyloliquefaciens subsp. plantarum MBI600 and its metabolites has been evaluated in iron deficiency conditions, in vitro and in planta, against the plant pathogens Pseudomonas syringae pv tomato and Pseudomonas syringae pv tabaci. In vitro assessment was based on formation of inhibition zones, inhibition of growth in broth media and growth kinetic analysis. Inhibition zones formed in agar plates were negatively correlated to iron concentration. Growth of pathogens in the presence of cell free culture filtrate (CFCF) of MBI600 cultures, grown in the presence of different iron concentrations, was observed to be reduced in a manner analogous to iron concentration. In planta assessment was held in tomato and N. benthamiana plants, following artificial inoculation with the pathogens and treatment with CFCF of MBI600 cultures grown in the presence of different iron concentrations. In the case of low iron concentration treatments, there is a statistically significant reduction in the total population of the pathogens in the leaf compared to the control treatment. Finally, iron starvation and siderophore production by MBI600 led to an antibiotic effect against the non-sensitive, under optimum iron diet conditions, Pseudomonas species. Finally, iron starvation upregulated lipopeptide synthase transcription (and thus possibly lipopeptide production) and enhanced competence traits such as swarming and chemotaxis.
Main subject category:
Science
Keywords:
Σιδηροφορείς(1), Bacillus amyloliquefaciens(2), Pseudomonas(3), φυτοπαθογόνα βακτήρια(4), συνθήκες έλλειψης σιδήρου(5).
Number of references:
120
