Interleukin 15 levels in follicular fluid and their effect on oocyte maturation

Postgraduate Thesis uoadl:2325669 492 Read counter

Unit:
ΠΜΣ Έρευνα στη Γυναικεία Αναπαραγωγή
Library of the School of Health Sciences
Deposit date:
2017-12-05
Year:
2017
Author:
Spanou Sofia
Supervisors info:
Μαστοράκος Γεώργιος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γαζούλη Μαρία, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Σφοντούρης Ιωάννης, Κλινικός Εμβρυολόγος
Original Title:
Διερεύνηση των επιπέδων της ιντερλευκίνης 15 στο ωοθυλακικό υγρό και της επίδρασης αυτών στην ωρίμαση των ωαρίων
Languages:
Greek
Translated title:
Interleukin 15 levels in follicular fluid and their effect on oocyte maturation
Summary:
This study was undertaken to determine the levels of IL-15 in follicular fluid (FF) and investigate their relation with oocyte maturity and secondarily with follicle size, as well as patients’ BMI and age. Follicular fluid specimens were obtained from 56 subfertile women undergoing Intracytoplasmic Sperm Injection (ICSI) during oocyte retrieval and IL-15 concentrations were calculated with ELISA. Wilcoxon’s test and Pearson’s correlation coefficient were used to correlate FF concentrations of IL-15 with follicular size and stage of oocyte maturation, along with patients’ BMI (Body Mass Index) and age. IL-15 concentrations in FF of follicles with immature oocytes were significantly higher than those from follicles with mature ones (median: 5,333 pg/ml vs. 3,250 pg/ml, respectively, p<0,001). There was also a significant negative correlation between IL-15 concentration and follicle size (r=-0,333, p=0,003). No correlation was observed between IL-15 concentrations and patients’ BMI and age. In conclusion, IL-15 follicular concentrations are adversely related with the maturity and size of the retrieved oocytes after ICSI. Follicular fluid IL-15 concentrations appear to be a promising predictive factor for oocyte competence.
Main subject category:
Health Sciences
Keywords:
Interleukin 15, Oocyte maturation, Cytokines, Follicular fluid
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
185
Number of pages:
86
Διερεύνηση των επιπέδων της ιντερλευκίνης 15 στο ωοθυλακικό υγρό και της επίδρασης αυτών στην ωρίμαση των ωαρίων.pdf (1 MB) Open in new window