Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότηταςLibrary of the School of Science
Author:
Antonopoulos Nikolaos-Nektarios
Supervisors info:
Ειρήνη Παντερή, Καθηγήτρια, Τομέας Φαρμακευτικής Χημείας, Τμήμα Φαρμακευτικής, Πανεπιστήμιο Αθηνών
Original Title:
Ανάπτυξη και επικύρωση μεθόδου υγροχρωματογραφίας υδρόφιλης αλληλεπίδρασης-φασματομετρίας μαζών (HILIC-ESI/MS)για τον ποσοτικό προσδιορισμό της μετφορμίνης και της ροσουβαστατίνης σε ανθρώπινο πλάσμα
Translated title:
Hydrophilic interaction liquid chromatography/mass spectometry method development and validation for the quantification of metformin and rosuvastatin in human plasma
Summary:
Metfomin is an anti-hyperglycemic agent used in patients with type 2 diabetes, reducing blood glucose. Rosuvastatin is an anti-lipidemic drug used in patients with dyslipidemia, reducing LDL cholesterol and tryglicerides. Due to the superiority in efficacy and other benefits, rosuvastatin and metformin are often prescribed together due to the high comorbidity of dyslipidemia and type 2 diabetes.
Main objective of the current study is HILIC-ESI/MS method development and validation for the quantification of the compounds mentioned above, in human plasma. The analysis is based in using 100 μl plasma, treated with acetonitrile – ammonium formate 5mM solution, leading to protein precipitation. After 20 min centrifuge, the samples are filtrated through 0.45μm Millipore PTFE filters and injected to the LC-ESI/MS system. The column, Xbridge HILIC (150.0 x 2.1mm i.d., particle size 3.5μm, 135Å), is used for the analytical separation of the analytes and the internal standard (N-Despropyl Ropinirole), through isocratic elution. A 12% 15.0 mM ammonium formate solution in acetonitrile is used as mobile phase, with 0,25 mL min-1 flow rate. The quantification of the analytes is achieved by ESI – SIM method, within 62.5 – 5000 ng mL-1 for metformin and 2,0 – 100 ng mL-1 for rosuvastatin.
The method was successfully applied for the analysis of plasma samples from patients administered one of the above compounds. In the future, the method could be used in clinical studies to monitor the levels of these drugs in patients and adjust their dosage.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
hydrophilic interaction liquid chromatography, LC-ESI/MS, metformin, rosuvastatin
