Study of the expression of CRH-R1 and CRH-R2 in the developments stage of blastocyst in mice

Postgraduate Thesis uoadl:2632218 334 Read counter

ΠΜΣ Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
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Kouvoutsaki Kouvoutsaki
Supervisors info:
Λουτράδης Δημήτριος, Καθηγητής, Ιατρική, ΕΚΠΑ
Δρακάκης Πέτρος, Αναπληρωτής καθηγητής, Ιατρική , ΕΚΠΑ
Καλλιανίδης Κωνσταντίνος, Αναπληρωτής καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Η μελέτη της έκφρασης των υποδοχέων της CRH, CRH-R1 και CRH-R2 σε βλαστοκύστεις ποντικών
Translated title:
Study of the expression of CRH-R1 and CRH-R2 in the developments stage of blastocyst in mice
Corticotropin – releasing hormone (CRH) is a 41 amino acid peptide, which is produced by the paraventicular nucleus of the hypothalamus and it is carried, through the portal system, to the anterior lobe of the pituitary, where it stimulates corticotropes. Two receptors have been found in human, CRH-R1 and CRH-R2, through whom CRH exerts its actions. CRH is located in adrenals, human lymphocytes and active inflammatory regions. In addition, CRH has been found in the uterus, the placenta, the ovaries, the trophoblast, the deciduas and the uterine glands. CRH produced by the deciduas and the trophoblast induces the secretion of FasL (pre- apoptotic cytokine). During the implantation of the blastocyst, the endometrium has inflammatory response characteristics, nevertheless, the embryo is not rejected as a semi-allograft it is. During this procedure the role of Fas/FasL pathway is in great importance. Due to the Fas/FasL pathway the excess of T cells is removed, through an inflammatory reaction, leading to the acceptance of the embryo.
The purpose of this thesis is the study of CRH receptors’ (CRH-R1, CRH-R2) expression in the developing stage of blastocyst in mice. In that way CRH action and importance in the implantation can be investigated.
For the study of CRH receptor’s expression the RNA extraction technique has been used. Afterwards cDNA was formed and used in the molecular technique of Real - Time PCR (RT-PCR). In that way, the expression of both receptors was measured, whereas the G6PD expression levels were used as a control for the study.
Main subject category:
Health Sciences
CRH receptors, Blostocysts, Implantation, Fas/FasL
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Kouvoutsaki Konstantina Master.pdf (3 MB) Open in new window