Distributioand immunoexpression of LOX, PCP and Myofibroblasts in Vaginal Tissue of Postmenopausal Women with and without Pelvic Organ Prolapse

Doctoral Dissertation uoadl:2682621 87 Read counter

Unit:
Τομέας Υγείας - Μητέρας - Παιδιού
Library of the School of Health Sciences
Deposit date:
2018-03-05
Year:
2018
Author:
Kleanthis Christos
Dissertation committee:
Παναγιώτης Βάκας, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Κωσταντίνος Πανουλής Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Ειρήνη Λαμπρινουδάκη, Αναπληρώτρια Καθηγήτρια, Ιατρική, ΕΚΠΑ
Ευθύμιος Κ. Δεληγεώρογλου, Καθηγητής, Ιατρική, ΕΚΠΑ
Λέων Αραβαντινός, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Θεόδωρος Πανοσκάλτσης, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Ματρώνα Φράγκου-Πλεμένου, Επίκουρη Καθηγήτρια, Ιατρική, ΕΚΠΑ
Original Title:
Κατανομή και μεταβολές των πρωτεϊνών της Λυσίλ Οξειδάσης (LysylOxidases, LOX) της πρωτεϊνάσης C του προκολλαγόνου (PCP) και των μυοϊνοβλαστών της εξωκυττάριας θεμέλιας ουσίας τοιχωμάτων κόλπου μεταμμηνοπαυσιακών γυναικών, με και χωρίς πρόπτωση
Languages:
Greek
Translated title:
Distributioand immunoexpression of LOX, PCP and Myofibroblasts in Vaginal Tissue of Postmenopausal Women with and without Pelvic Organ Prolapse
Summary:
ABSTRACT
AIM
Scopeof the study was to investigate the difference of distribution and expression of
the lysyl oxidase enzymes (LOX), the pro-collagen C proteinase (PCP) and the
myofibroblasts in the vaginal tissue of post-menopausal women, with and without
prolapse.
MATERIALS and METHODS
This was a prospective closely matched controlled clinicopathological study. Samples
were obtained from the anterior vaginal wall of postmenopausal patients that
underwent hysterectomy for benign conditions. The immunohistochemical study of the
samples was performed on paraffin embedded specimens with the semi-automatic
Ventana method according to the manufacturer’s instructions. We have also examined
the correlation between prolapse and arity, age and body weight in the same
population.
RESULTS
We have examined vaginal tissue from 44 postmenopausal patients: 21 with prolapse
and 23 without (control group). LOX enzymes were observed in all samples, but they
were strongly expressed only in the control group (P<0.005). Similarly PCP and
myofibroblasts were positive in all tissues but no statistically important difference in
their expression was observed between the two groups. Linear correlation between
older age and higher parity with prolapse was observed. There was no correlation
between prolapse and body weight.
CONCLUSION
LOX enzymes are strongly expressed in the vaginal tissue of postmenopausal women
without prolapse and may play an important role in the pathophysiology of POP.
There is no statististical difference in the expression of PCP and fibroblasts between
the control group and the patients with prolapse. Higher parity and older age are
associated with prolapse, but the same doesnot apply for higher body weigh.
Main subject category:
Health Sciences
Keywords:
LOX - Lysyl Oxidases, PCP - Procollagen C Proteinase, Immunoexpression, Vaginal wall, Prolapse, Myofibroblast, Actin, Menopause
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
251
Number of pages:
134
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