METHODS DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF PHENOL AND M-CRESOL IN PORK TISSUE BY LC-MS AND GC-MS

Postgraduate Thesis uoadl:2694332 348 Read counter

Unit:
Κατεύθυνση Χημική Ανάλυση-Έλεγχος Ποιότητας
Library of the School of Science
Deposit date:
2018-03-10
Year:
2018
Author:
Manousi Natalia
Supervisors info:
Ιωάννης Παπαδογιάννης, Καθηγητής, Τμήμα Χημείας, Α.Π.Θ.
Original Title:
ΑΝΑΠΤΥΞΗ ΚΑΙ ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΩΝ ΓΙΑ ΤΟΝ ΤΑΥΤΟΧΡΟΝΟ ΠΡΟΣΔΙΟΡΙΣΜΟ ΦΑΙΝΟΛΗΣ ΚΑΙ Μ-ΚΡΕΣΟΛΗΣ ΣΕ ΙΣΤΟ ΔΕΡΜΑΤΟΣ ΧΟΙΡΟΥ ΜΕ ΤΙΣ ΤΕΧΝΙΚΕΣ LC-MS ΚΑΙ GC-MS.
Languages:
Greek
Translated title:
METHODS DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF PHENOL AND M-CRESOL IN PORK TISSUE BY LC-MS AND GC-MS
Summary:
In this master thesis, two derivatives of benzene, phenol and m-cresol were
studied. Phenol is a colorless volatile crystalline solid, with a characteristic sweet and
bitter odor and a very caustic taste, slightly soluble in water and hygroscopic. In
addition, it is poisonous and mutagenic and can directly cause white spots and
chemical burns on the skin. Due to its antiseptic and disinfecting action, it is used in
the manufacture of cosmetics, such as hair dyes, sunscreen and skin care products.
Accordingly, m-cresol is a colorless, viscous liquid used both as a biocide /
preservative and as a flavoring agent in products for skin care, personal care products,
bath and hair products, and oral solutions. These compounds can harm humans, when
they are present in cosmetic products.
The aim of this study was to determine the above mentioned compounds in pork
skin tissue, which is the most appropriate model for simulating human skin due to the
similarities they have in surface properties, body mass, surface area ratio body, skin
rejuvenation kinetics, hair follicular density, permeability and lipid build-up.
The determination was accomplished by liquid chromatography in combination
with mass spectrometry after atmospheric pressure chemical ionization of the
compounds (LC-APCI-MS), as well as by gas chromatography technique combined
with mass spectrometry (GC-MS), after derivatization of the compounds.
The two developed methods were compared with regards to their performance
characteristics and the sample preparation procedure. The validation of the methods
was carried out using spiked pork skin tissue samples in terms of linearity, recovery,
limits of detection and quantification, precision, repeatability and accuracy. The
results were comparable, demonstrating that the determination of phenol and m-cresol
for routine analysis can be successfully performed either by liquid or by gas
chromatography.
The main difference between the two methods is the additional derivatization step
required for gas chromatography, which extends the experimental procedure by 30
min. This step is a necessary because of the low volatility of the two phenolic
compounds, which makes it difficult to determine them by gas chromatography.
In conclusion, the developed methods are simple, economical, accurate and
repeatable and they can be used to determine phenol and m-cresol in pork skin tissue
145
samples. The choice between the two methods can be based on the available
instrumentation of the laboratory and the time needed for executing the experiment.
Main subject category:
Science
Keywords:
phenol, m-cresol, lc-ms, hplc, gc-ms, pork tissue
Index:
No
Number of index pages:
0
Contains images:
No
Number of references:
45
Number of pages:
148
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