Unit:
Κατεύθυνση Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Author:
Kotsaridis Konstantinos
Supervisors info:
Γεώργιος Διαλλινάς-Καθηγητής-Τμήμα Βιολογίας-Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Δημήτριος Χατζηνικολάου-Αναπληρωτής Καθηγητής-Τμήμα Βιολογίας-Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Κοσμάς Χαραλαμπίδης-Επίκουρος Καθηγητής-Τμήμα Βιολογίας-Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Μελέτη της αλληλεπίδρασης του ιού Υ της πατάτας με το φυτό-ξενιστή πιπεριά
Translated title:
Study of the interactions between Potato virus Y and its pepper host plant
Summary:
The control of plant viral diseases is mainly based on prevention by using genetically resistant plants. However, due to the plasticity of their genomes, viruses can overcome plant resistance and even invade new plant species. The protein-protein interaction network between viral and plant proteins seems to be crucial in such host-jump events. Recently it was shown that P3 genetic region of Potato virus Y (PVY, genus Potyvirus) is responsible for PVY adaptation in pepper plants. Moreover, this region encodes for two proteins, P3 and P3N-PIPO, through transcriptional slippage of the viral RNA polymerase. To investigate the interactions that are possibly involved in PVY adaptation in pepper plants, plant proteins that can physically interact with potyviral P3N-PIPO and determine their systemic movement in many pathosystems were identified. This study focuses on PCaP1 (Plasma membrane Cation binding Protein 1) protein that was recently shown to be involved in intercellular movement of Turnip mosaic virus (TuMV, genus Potyvirus) in arabidopsis. The orthologous PCaP1 of pepper was identified, sequenced and further examined for its putative role in PVY movement through virus induced gene silencing (VIGS). The experimental approaches at plant level confirmed the involvement of PcaP1 in systemic movement of PVY.
Main subject category:
Science
Keywords:
Potato virus Y (PVY)(1), plant-host pepper(2), PCaP1 protein(3), P3N-PIPO protein(4), virus induced gene silencing (VIGS)(5)
Number of references:
116
