Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότητας
Library of the School of Science

2018-04-22

2018

Papaefstathiou Efstathios

Ειρήνη Παντερή, Καθηγήτρια, Τμήμα Φαρμακευτικής, Ε.Κ.Π.Α.

Συγκριτική μελέτη υγροχρωματογραφίας κατανομής αντίστροφης φάσης με υγροχρωματογραφία υδρόφιλης αλληλεπίδρασης για τη σάρωση διογκωτικών πλάσματος σε ούρα αθλητών με αναλυτή μαζών χρόνου πτήσης (LC-ESI/qTOF/MS)

Greek

Comparative study of reversed phase liquid chromatography (RPLC) with hydrophilic interaction chromatography (HILIC) for the detection of plasma volume expanders in athlete's urine with time of flight mass spectrometry (LC-ESI/qTOF/MS)

This Master Thesis presents the development and validation of a screening method in order to detect Plasma Volume Expanders (PVEs) - Dextran and Hydroxyethyl starch – in athlete’s urine with Reversed Phase Liquid Chromatography - Mass Spectrometry and the use of a Time of Flight mass analyzer coupled with a quadrupole (LC-qTOF) with positive electronspray ionization (+ESI), compared to Hydrophilic Interaction Liquid Chromatography – Mass Spectrometry. At first, they were selected the ions for the identification of the two analytes and studied their chromatographic behavior in both reversed phase liquid chromatography and hydrophilic interaction chromatography. Then, a method validation was performed for both methods in accordance with the Specification and the Standardized Work Format of Doping Control Laboratory,
As a result, both of the methods being proposed were considered to be capable of detecting hydroxyethyl- starch and dextran, but hydrophilic interaction chromatography has shown better chromatographic behavior and less matrix interferences rather than reversed phase liquid chromatography.

Science

doping. dextran, hes, liquid chromatography

Yes

4

Yes

64

82

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https://pergamos.lib.uoa.gr/uoa/dl/object/2748041