Study of the antifungal activity of analogous natural metabolites from algae and plants

Postgraduate Thesis uoadl:2750940 305 Read counter

Unit:
Κατεύθυνση Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2018-05-15
Year:
2018
Author:
Tsouvali Paraskevi
Supervisors info:
Γεώργιος Διαλλινάς, Καθηγητής Μοριακής Μικροβιολογίας, Τμήμα Βιολογίας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Αμαλία Καραγκούνη, Καθηγήτρια Τμήμα Βιολογίας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Δημήτριος Χατζηνικολάου, Επικ. Καθηγητής Τμήμα Βιολογίας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Μελέτη της αντιμυκητιακής δράσης αναλόγων φυσικών μεταβολιτών από φύκη και φυτά
Languages:
Greek
Translated title:
Study of the antifungal activity of analogous natural metabolites from algae and plants
Summary:
Fungi are a group of eukaryotic microorganisms found on the ground, in the air and in the water. They have the ability to infect many plants, animals but also humans. In particular, aspergillosis is a widespread disease caused by species of the genus Aspergillus. Drugs have been developed to treat infections caused from these fungi. The inappropriate use of antifungal drugs, combined with the inherent capacity of some fungi to resist these formulations, has led to the selection of resistant fungal strains. The production of new pharmacological formulations based on natural analogues of plant origin could address the problem of resistance to existing antifungal medicines. The purpose of the present thesis is the discovery of new chemical substances of plant origin with antifungal activity. Natural metabolites from the algae Laurencia and chemical analogues of tyrosol from Olea europaea were tested under controlled environmental conditions in order to examine their antifungal properties and to determine their toxicity specifically in strains of A. nidulans, A. fumigatus and A. flavus. Furthermore, in order to find the mechanism of action of slected active substances, fluorescence microscopy experiments and kinetic studies were performed. Additionally, mutagenesis experiments were carried out to ascertain how likely is the recovery of resistance to these substances in mutant strains of A. nidulans. Finally, an experiment was conducted to determine the toxicity in mammalian cells, the results of which are critical for the development of safe novel antifungals.
Main subject category:
Science
Other subject categories:
Biology
Biomedical Engineering
Keywords:
Antifungal activity, Fungi, Algae, Olea europaea , Natural metabolites
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
132
Number of pages:
86
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