Unit:
Κατεύθυνση Ενδοδοντία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Kontogiannis Taxiarchis
Supervisors info:
Νικόλαος Κερεζούδης, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Κωνσταντίνος Τόσιος, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Παναγιώτης Πανόπουλος, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Original Title:
Μελέτη της επίδρασης του υδροξειδίου ασβεστίου στην απόπτωση των επιθηλιακών κυττάρων ακρορριζικών κύστεων
Translated title:
Effect of calcium hydroxide as intracanal medicament on the apoptosis of radicular cyst epithelium
Summary:
Introduction: Apoptosis is a genetically-determined programmed cell death. Two basic apoptotic pathways exist; one of them seems to be regulated by calcium ions, resulting in the activation of caspase-9. Although apoptosis indeed takes place in the radicular cyst epithelium, and calcium ions might be present in the lesion as exogenous material via extrusion, it has not been possible to be correlated with any clinical factor.
Aim: To investigate the effect of calcium hydroxide (Ca(OH)2) as intracanal medicament on the apoptosis of radicular cyst epithelium, via the immunohistochemical detection of caspase-9.
Materials & methods: 42 paraffin-embedded radicular cysts were retrospectively collected. Information about gender and age of the patients, the size of each cyst (expressed by its maximum diameter) and whether Ca(OH)2 had been applied as intracanal medicament before the surgical treatment were collected from the clinical histories. All cases had been followed-up for 6-12 months after completion of conservative treatments (regardless of whether Ca(OH)2 had been applied during treatment or not). All apicoectomies were carried out after this follow-up period. Sections from all cysts were stained with the von Kossa technique for the detection of exogenous calcium deposits. Three groups were formed: (a) single-visit treatments with no calcium deposits (n=14), (b) multiple-visit treatments without calcium deposits (n=12), (c) multiple-visit treatments with calcium deposits, corresponding to hydroxide extrusion (n=16). All cysts were then immunohistochemically stained for caspase-9 to record apoptosis of the epithelium. Statistical analysis followed.
Results: The frequency of caspase-9 immunoreactivity in the cystic epithelium in the three groups was 42.86%, 50% & 93.75% of cysts, respectively. In the two hydroxide groups, immunoreactivity was significantly more frequent (p=0.04<0.05), particularly in the group with extrusion (p=0.007<0.05). In cysts larger than (or equal to) 10mm, caspase-9 was more frequently expressed, irrespective of whether Ca(OH)2 had been applied or not (p=0.004<0.05).
Conclusion: Calcium hydroxide up-regulates the expression of caspase-9 and therefore apoptosis; this is more intense when it is extruded into the lesion and especially in large lesions.
Main subject category:
Health Sciences
Keywords:
Radicular cyst, Epithelium, Apoptosis, Calcium hydroxide, Caspases
Number of references:
300
