Unit:
Κατεύθυνση Σχεδιασμός και Ανάπτυξη νέων Φαρμακευτικών Ενώσεων - Φαρμακευτική ΧημείαLibrary of the School of Science
Author:
Kyriazi Artemis-Zoi
Supervisors info:
Κωστάκης Ιωάννης, Επίκουρος Καθηγητής, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Γκίκας Ευάγγελος, Επίκουρος Καθηγητής, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
«Σύνθεση, υπολογιστική μελέτη και αξιολόγηση νέων αντιδραστηρίων παραγωγοποίησης για ανίχνευση και ποσοτικό προσδιορισμό πρωτοταγών αμινών»
Translated title:
"Synthesis, theoritical studies and evaluation of novel derivatization reagents for the detection and quantification of primary amines"
Summary:
Derivatization is a technique widely used in Analytical Chemistry and Pharmaceutical Analysis, mainly due to the ability to perform detection and quantitation in unstable compounds. The improved chromatographic behavior of the analyte and the increased traceability are some other advantages of this technique. By selecting a fluorescent derivatization reagent, taking advantage of the fluorescence effect, sensitivity to detection and quantification is further enhanced. In the present thesis, 1-bromomethyl-2-nitro-9H-xanthen-9-one and its analogues are synthesized, a derivatization reagent for primary amines. The important advantage of this reagent is that it does not fluoresce, while the derivatization product has intense fluorescence, eliminating the possibility of inhibiting the measurement due to overlapping reagent-product signals. Experimental evaluation of the derivatives resulting from reaction with 2-butylamine, computational study of reagents and derivatives, with respect to reactivity and photochemical properties respectively, and comparison of experimental and theoretical results are followed. Finally, using Multivariate Analysis, a model for prediction of photochemical properties of potential derivatization products is derived.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
derivatization, primary amines, fluorescence
