Appraisal of the Intensinal Absorption of Flavonoids using Reversed Phase Chromatography: Evaluation of the static phase "Discovery RP Amide C16."

Postgraduate Thesis uoadl:2766615 244 Read counter

Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότητας
Library of the School of Science
Deposit date:
2018-06-04
Year:
2018
Author:
Koutropoulou Nikolia
Supervisors info:
Γιαγκίνης Κωνσταντίνος, Επίκουρος Καθηγητής Φυσιολογίας του Ανθρώπου, Τμήμα Επιστήμης Τροφίμων και Διατροφής, Σχολή περιβάλλοντος, Πανεπιστήμιο Αιγαίου
Original Title:
Αξιολόγηση της Εντερικής Διαπερατότητας Φλαβονοειδών με τη χρήση Αντιστρόφου Φάσεως Χρωματογραφία: Αξιολόγηση της στατικής φάσης "Discovery RP Amide C16".
Languages:
English
Greek
Translated title:
Appraisal of the Intensinal Absorption of Flavonoids using Reversed Phase Chromatography: Evaluation of the static phase "Discovery RP Amide C16."
Summary:
Flavonoids belong to the chemical class of polyphenols, being found in large quantities in plants and exhibiting a variety of medicinal properties. In particular, many flavonoids have antioxidant, anti-atherosclerotic, hepato-protective and anti-inflammatory effects, as well as anti-tumor properties. The absorption of flavonoids through small intestine mucosa is considered to be a critical parameter for the manifestation of their biological activity. Lipophilicity significantly affects the absorption of xenobiotic substances through the intestinal epithelium.
In the present study, the chromatographic logkw indices of 33 flavonoids were determined by the use of reverse phase high performance liquid chromatography (RP-HPLC). Discovery RP Amide C16 reverse phase column was used as static phase. For the preparation of mobile phase, octanol-saturated MOPS buffer with pH 7.4 was used aqueous component and methanol as organic modifier. A large number of physicochemical properties of flavonoids were determined using software programs. The intestinal permeability data of the flavonoids studied were collected from the literature.
A similar elution mechanism was found for all flavonoids, excluding their glycosides, based on the strong linear logkw / S relationship. A satisfactory linear correlation was observed between logkw indices and intestinal permeability coefficient for 20 flavonoids for which bibliographic data was available. The correlation recorded was better than the corresponding linear correlations derived by the used of immobilized artificial membrane columns for the under study data set. The introduction of S and V terms significantly improved the correlation between logkw indices and intestinal permeability coefficient. The resulting final equation could be used to estimate the intestinal permeability of flavonoids for which no experimental values are available in the literature.
Main subject category:
Science
Keywords:
Flavonoids, Reverse Phase High Performance Liquid Chromatography (RP-HPLC), Lipophilicity, Intestinal Absorption, Caco-2 cells, Discovery RP Amide C16
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
121
Number of pages:
84
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