In vitro study of CSF from patients with traumatic brain injury in stem cells cultures

Doctoral Dissertation uoadl:2766617 149 Read counter

Τομέας Χειρουργικής
Library of the School of Health Sciences
Deposit date:
Androulis Antonios
Dissertation committee:
Εμμανουήλ Λέανδρος, Καθηγητής, Ιατρική, ΕΚΠΑ
Ευστάθιος Μποβιάτσης, Καθηγητής, Ιατρική, ΕΚΠΑ
Μανούσος Κωνσταντουλάκης, Καθηγητής, Ιατρική, ΕΚΠΑ
Κωνσταντίνος Βουμβουράκης, Καθηγητής, Ιατρική, ΕΚΠΑ
Γεώργιος Τσιβγούλης, Καθηγητής, Ιατρική, ΕΚΠΑ
Γεώργιος Δημόπουλος, Αναπληρωτής Καθηγητής, Ιατρική , ΕΚΠΑ
Στέφανος Κορφιάς, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Μελέτη της invitro επίδρασης ΕΝΥ ασθενών με κρανιοεγκεφαλική κάκωση σε καλλιέργειες βλαστοκυττάρων
Translated title:
In vitro study of CSF from patients with traumatic brain injury in stem cells cultures
Traumatic brain injury (TBI) is a complex condition that presents with a wide spectrum of clinical symptoms caused by an initial insult to the brain through an external mechanical force to the skull. TBI is one of the leading causes of mortality among young adults in the developed world. Pathophysiology of TBI is complex and consists of acute and delayed injury. In the acute phase, brain tissue destroyed upon impact includes neurons,glia and endothelial cells, the latter of which makes up the blood-brain barrier. In the delayed phase, “toxins” released from damaged cells set off cascades in neighboring cells eventually leading to exacerbation of primary injury. As researches further explore pathophysiology and molecular mechanisms underlying this debilitating condition, numerous potential therapeutic strategies, especially those involving stem cells, are emerging to improve recovery and possibly reverse damage.
Mesenchymal stem cells (MSCs) have been regarded for their clinical therapeutic potential for central nervous system (CNS) pathologies. Their potential utility is a result of their intrinsic ability to repair damaged tissues, deliver therapeutic proteins, and migrate to sites of pathology within the brain. However, it remains unclear whether the CNS promotes any changes in these potential therapeutic cells, which would be critical to understand before clinical application. A major component of the CNS is cerebrospinal fluid (CSF).
Therefore, the aim of this study was to evaluate the influence that human CSF has on the function of human adipose-derived MSCs (hAMSCs) in regard to cell proliferation, survival, and migration. Furthermore, the CSF and Blood levels of EMMPRIN and Osteopontin, estimated in TBI and neurological patients, as prognostic indicators.
Main subject category:
Health Sciences
Stem cells, Emmprin, Osteopontin, Biomarkers, Traumatic brain injury
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