ΠΜΣ Νεοπλασματική Νόσος στον Άνθρωπο: Σύγχρονη Κλινικοπαθολογοανατομική Προσέγγιση και Έρευνα
Library of the School of Health Sciences

2018-06-22

2018

Tzoumerkioti Theodora

Ανδρέας Χ. Λάζαρης, Καθηγητής, Ιατρική, ΕΚΠΑ
Νικόλαος Καβαντζάς, Καθηγητής, Ιατρική, ΕΚΠΑ
Ειρήνη Θυμαρά, Επίκουρη Καθηγήτρια, Ιατρική, ΕΚΠΑ

Εφαρμογές χρωμογόνου in situ υβριδισμού (CISH) σε καρκινικούς ιστούς

Greek

In situ chromogenic hybridization (CISH) applications in cancer tissues

Neoplasms are a group of abnormal cells that form lumps or growths with a genetic basis. The gene alteration, which the cells undergo after the influence of various external factors, lead to cancer cells formation. Thus, in most neoplasms, defective copies of genes that are directly involved in cell division are detected and leads to uncontrolled tumor proliferation and abnormal differentiation in normal cells.
Cancer modern genetic approach requires the identification of molecular prognostic and predictive markers. The evaluation of those markers (DNA, RNA, proteins) allows the molecular classification of cancer and gives us information about the appropriate treatment that patients should follow. One of the most well-known markers is overexpression of the HER2 gene as it contributes to the development of the disease and represents an attractive therapeutic target. As a result of the importance of estimating HER2, several molecular methods have been described to evaluate its condition.
The in situ hybridization technique (FISH, SISH and CISH) is considered to be method of choice for determining the numerical and structural characteristics of the genetic material and its lesions, expressed in tumour. For this reason, this technique is a valuable both research and diagnostic tool for cancer patients. This is a fairly sensitive method and because of this all steps, from receiving the sample in the laboratory up to delivery to the pathologist need to be done with particular care for the determination of gene expression. For this reason, the hybridization steps and the technique of CISH hybridization in specimens brought up by biopsy are presented in detail for full understanding. In addition, a reference is made to fluorescence (FISH) and to silvering hybridization (SISH) chromogen as well and comparing to CISH chromogenic hybridization.
Relying on medical research the above molecular techniques aims at early diagnosis, predicting the development of the disease as well as choosing the appropriate treatment.

Health Sciences

Chromogenic, Cancer, Method

No

0

Yes

89

63

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https://pergamos.lib.uoa.gr/uoa/dl/object/2775310