Development and Validation of HPLC Methods for Quantitative Determination and Impurity Testing in Travoprost-Timolol Eye Drops

Postgraduate Thesis uoadl:2775489 248 Read counter

Unit:
Κατεύθυνση Αναλυτική Χημεία
Library of the School of Science
Deposit date:
2018-06-25
Year:
2018
Author:
Magkiosi Konstantina
Supervisors info:
Κουππάρης Μιχαήλ, Καθηγητής Τμήμα Χημείας ΕΚΠΑ
Original Title:
Ανάπτυξη και Επικύρωση Μεθόδων HPLC για Ποσοτικό Προσδιορισμό και Έλεγχο Προσμίξεων σε Διάλυμα Οφθαλμικών Σταγόνων Travoprost-Timolol
Languages:
Greek
Translated title:
Development and Validation of HPLC Methods for Quantitative Determination and Impurity Testing in Travoprost-Timolol Eye Drops
Summary:
The purpose of this MSc thesis was the selection, development and validation of methods for the determination of active Travoprost, active Timolol, impurities of Travoprost, impurities of Timolol in a Travoprost -Timolol eye drop solution, and validation for the determination of the excipient benzalkonium chloride using High Performance Liquid Chromatography technique.
The isocratic HPLC method of USP was chosen for the determination of Travoprost and its cleavage products. A method of gradient elution of pairs of ions was developed to determine Timolol and its impurities. A gradient elution method for Travoprost related compound A was developed. To determine the benzalkonium chloride excipient was selected by HPLC isocratic elution.
During method validation, appropriate quqlity characteristics were investigated, such as linearity, precision, accuracy, robustness and limits of detection-quantification. The evaluation of the above characteristics for each method was performed according to international guidelines. All methods were found appropriate for the purpose for which they are intended.
Main subject category:
Science
Keywords:
Travoprost-Timolol, HPLC, impurities, eyedrops, assays
Index:
Yes
Number of index pages:
6
Contains images:
Yes
Number of references:
21
Number of pages:
129
File:
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