VALIDATION OF A GC/HRMS METHOD FOR QUANTITATIVE DETERMINATION OF 19-NORADROSTERONE AND 19-NORETIOCHOLANOLONE GLYCURONIDES, AND THEIR RATIO, AT ATHLETES URINE.

Postgraduate Thesis uoadl:2775509 324 Read counter

Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότητας
Library of the School of Science
Deposit date:
2018-06-25
Year:
2018
Author:
Mixalaki Afroditi
Supervisors info:
Ε. ΠΑΝΤΕΡΗ Καθηγήτρια ΕΚΠΑ
Ι. ΛΟΥΚΑΣ Αναπληρωτής Καθηγητής ΕΚΠΑ
Ι. ΝΤΟΤΣΙΚΑΣ Επίκουρος Καθηγητής ΕΚΠΑ (Επιβλέπων)
Original Title:
ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΟΥ GC/HRMS ΓΙΑ ΤΟΝ ΠΟΣΟΤΙΚΟ ΠΡΟΣΔΙΟΡΙΣΜΟ ΤΩΝ ΓΛΥΚΟΥΡΟΝΙΔΙΩΝ ΤΗΣ 19-ΝΟΡΑΝΔΡΟΣΤΕΡΟΝΗΣ ΚΑΙ ΤΗΣ 19-ΝΟΡΕΤΙΟΧΟΛΑΝΟΛΟΝΗΣ ΚΑΙ ΤΟΥ ΛΟΓΟΥ ΤΟΥΣ ΣΕ ΟΥΡΑ ΑΘΛΗΤΩΝ
Languages:
Greek
Translated title:
VALIDATION OF A GC/HRMS METHOD FOR QUANTITATIVE DETERMINATION OF 19-NORADROSTERONE AND 19-NORETIOCHOLANOLONE GLYCURONIDES, AND THEIR RATIO, AT ATHLETES URINE.
Summary:
The purpose of this Master Thesis was the validation of a GC/HRMS method for the detection and quantification of two metabolites of synthetic anabolic Nadrolone, glucuronides of 19-norandrosterone and 19-norcholanolone, but also their ratio.
Based on the World Anti-Doping Agency guidelines, each accredited laboratory must have a validated method for quantifying the ratio of the two metabolites in cases where it can not be determined whether it was an endogenous or exogenous origin of 19-norandrosterone glucuronide. If the ratio of the two metabolites (19-NA / 19-NE) is less than 3 then it is due to endogenous production.
For the sample processing liquid-liquid extraction was performed. The analysis of the samples was performed by with a gas chromatographic system combined with the mass spectrometer detector (GC / HRMS). Analytes were ionized with an electron ionization (electron impact, (+) EI).
For the evaluation and validation of the method, the relative retention time, linearity, specificity, sensitivity variance, limit of detection and quantification for each of the two substances, the intermediate precision of the method, the repeatability, the carry over, and method uncertainty.
In conclusion, the method was assessed as adequate and appropriate for the purpose for which it was developed.
Main subject category:
Science
Other subject categories:
Pharmacy
Keywords:
Method validation, GC/HRMS, 19-norandrosterone, 19-noretiocholanolone, anabolic androgenic steroids
Index:
Yes
Number of index pages:
3
Contains images:
Yes
Number of references:
45
Number of pages:
123
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