Unit:
Κατεύθυνση Κοσμητολογία - ΔερματοφαρμακολογίαLibrary of the School of Science
Author:
Aritzidou Konstantina-Maria
Supervisors info:
Δάλλας Παρασκευάς, Επίκουρος Καθηγητής στον Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής
Ράλλης Μιχαήλ,Επίκουρος Καθηγητής στον Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής
Ευάγγελος Κάραλης, Επίκουρος Καθηγητής στον Τομέα Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής
Original Title:
Μορφοποίηση ημιστερεών σκευασμάτων Ρεσβερατρόλης, εκτίμηση της in vitro διαπερατότητας και της in vivo αντιφλεγμονώδους δράσης
Translated title:
Formulation of semisolid products of Resveratrol, evaluation of the in vitro transdermal delivery and the in vivo anti-inflammatory action
Summary:
In the present study, resveratrol permeability in vitro was studied in Franz cells as well as the effectiveness of its topical anti-inflammatory action in vivo in hairless mice type SKH-1. At the same time, a correlation bettween the in viro permeability and the in vivo efficacy was performed.
The objective of the in vitro permeation experiments was to enhance the permeability of resveratrol but also to evaluate a number of factors influencing permeability such as substance concentration, thermodynamic activity, carrier composition and the effect of a penetration enhancer. For this purpose preliminary experiments were performed on both STRAT-M synthetic membrane and human cadaver epidermis, where resveratrol gels with increasing concentration (1%, 2.5% and 5% saturation concentrationd) were used. It was observed that the gel with 2.5% resveratrol concentration showed optimal percutaneous permeability. Subsequently, using the vehicle of this formulation, an attempt was made to enhance the resveratrol permeability using Transcutol® (ethylene glycol monoethyl ether) as a permeation enhancer. In the new formulas,Transcutol ® was used in two concentrations (5% and 10%) and resveratrol at saturation concentration(5,3% and 8,1% respectively). The use of the enhancer, in both concentrations enhanced the permeability significantly in comparison to the non-enriched formula. The highest permeability was sustained when the enhancer was used at a concentration of 5% , and the results where statistically significantly different than those of the concentration of 10%.
After completing the in vitro experiments, an in vivo experiment was conducted in order to assess the anti-inflammatory action of resveratrol but also the effect of the permeation enhancer and thermodynamic activity on clinical efficacy. For this purpose, inflammation was caused on the skin of hairless by UV irradiation and the 3 resveratrol formulations that had yielded the better results of the in vitro permeation experiment were applied. Resveratrol appeared to have a satisfatory anti-inflammatory effect as the control group pf mice presented a significantly more intense inflammation than those treated with a resveratrol formulation. Regarding the correlation between the in vitro and the in vivo experiments, it appeared that the formulation that presented the optimum permeability was the one that gave the optimal anti-inflammatory efficacy as well.
Main subject category:
Health Sciences
Other subject categories:
Pharmacy
Pharmaceutical technology
Keywords:
Formulation, Resveratrol, Transdermal, Delivery, Franz, Anti-inflammatory, in vitro, in vivo
Number of index pages:
218
