Unit:
Κατεύθυνση Βιομηχανική ΦαρμακευτικήLibrary of the School of Science
Author:
Kotroni Eleftheria
Supervisors info:
Επιβλέπων Καθηγητής: Μιχαήλ Ράλλης, Επίκουρος Καθηγητής, Τομέας Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας, Εθνικόν και Καποδιστριακόν Πανεπιστήμιο Αθηνών
Συνεπιβλέπων Καθηγητής: Βασίλειος Ρούσσης, Καθηγητής, Τομέας Φαρμακογνωσίας και Χημείας Φυσικών Προϊόντων, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας, Εθνικόν και Καποδιστριακόν Πανεπιστήμιο Αθηνών
Παρασκευάς Δάλλας, Επίκουρος Καθηγητής, Τομέας Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας, Εθνικόν και Καποδιστριακόν Πανεπιστήμιο Αθηνών
Original Title:
Θεραπευτική Προσέγγιση της Φλεγμονής του Δέρματος με Επιθέματα
Translated title:
" Skin anti-inflammatory treatment with topical patches "
Summary:
Skin inflammation is associated with many diseases and skin disorders. Inflammation caused by ultraviolet radiation on normal skin and its treatment by various topical anti-inflammatory agents has been widely studied. However, treatment by topical patches remains largely unexplored.
In this study, nanofibrous polymeric patches of five different compositions were prepared by electrospinning and were studied for their anti-inflammatory activity on mice.
The experimental study was divided into two parts. Initially, electrospinning of polymeric patches that were subsequently evaluated for their anti-inflammatory activity took place. Three types of patches composed of Sodium Alginate (SA), Cellulose Acetate (CA), Polyethyleneoxide (PEO), and aqueous extract of Pinus halepensis were prepared at different percentages. In addition, patches of SA, CA and PEO, as well as patches of CA and PEO were electrospun. For the Control mice group a simple, sterile gauze of the same size as the other patches was used.
In the second part, the study of the anti-inflammatory effect of the produced patches took place. In particular, a single dose of ultraviolet radiation (3 M.E.D.) was initially applied to SKH-1, hairless, female mice causing a skinburn. Each in vivo study lasted 10 days, the first one consisting of 4 groups of 6 female mice (P. halepensis 8.2% w/w) and the second one consisting of 5groups of 6 female mice (P. halepensis 4.25% w/w and 15.1% w/w). A patch was applied for 24 hours to each mice of the group. During the experiment, the parameters of TEWL (transepidermal water loss), hydration, sebum, redness, thickness and elasticity of the skin were also measured. On the tenth and final day after the end of the experiment, the animals were terminated and skin sections of the irradiated dorsal skin were used for histopathological observations.
The results were very promising, confirming the anti-inflammatory effect of P. halepensis extract, as the group treated with the patches containing the highest percentage showed significant anti-inflammatory activity and maintenance of the normal skin properties, when compared to the other groups. The above conclusions were based on the everyday clinical condition, the histopathological examination and the statistical analyses of the skin parameters measured.
Main subject category:
Science
Keywords:
Skin, Inflammation, Skinburn, patches, Electrospinning, Pinus Halepensis extract
Number of references:
187
