Coagulation disorders in colon cancer

Postgraduate Thesis uoadl:2778804 382 Read counter

Unit:
ΠΜΣ Θρόμβωση-Αιμορραγία-Ιατρική των Μεταγγίσεων
Library of the School of Health Sciences
Deposit date:
2018-07-17
Year:
2018
Author:
Simos Nikolaos
Supervisors info:
Μαριάννα Πολίτου, Αναπληρώτρια Καθηγήτρια, Ιατρική, ΕΚΠΑ
Σερένα Βαλσάμη, Επίκουρη Καθηγήτρια, Ιατρική, ΕΚΠΑ
Δημήτριος Δημητρούλης, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Διαταραχές της αιμόστασης στον καρκίνο του παχέος εντέρου
Languages:
Greek
Translated title:
Coagulation disorders in colon cancer
Summary:
Cancer cells can release pre-hepatic tissue factor and micro-cells (MP) that can directly activate the coagulation cascade. The tumor cells actively release MPs but also promote the formation of platelet MPs. However, hypercoagulation is a pathological condition of the haemostatic mechanism that is difficult to detect with conventional coagulation tests in cancer patients. Thromboelastometry is a sensitive method that is able to show signs of hypercoagulability in the form of graphs, which can not be detected by classical laboratory tests of hemostasis. The viscoelastic properties of the thrombus created in a thrombelostrometry assay and the ability of the ROTEM analyzer to record them in the form of a graph is the principal helper of the laboratory in understanding and plotting a pathological-hypercoagulant condition of the haemostatic mechanism. Thromboelastometric measurements in cancer patients show significant evidence of hypercoagulation in relation to healthy controls. Colon cancer patients, especially advanced, also exhibit increased levels of platelet microparticles (PMPs) and other origins. The aim of our study was to investigate the role of MPs in in-vitro thrombus formation as depicted in a thrombo-elastometry chart in patients with colon cancer. Thrombocytometric assays were performed in platelet-poor plasma samples to demonstrate the possible haemostatic role of the microcirculants in colorectal cancer patients. High-count microparticle samples were selected to evaluate the contribution of microparticles - mainly of platelet origin - to the formation of the thrombus as shown in a thromboelastometry chart. Fibrinogen, as biologically expected is related to the CFT, angle and MCF parameters. Elevated fibrinogen and positive D-dimers probably indicate a continuous activation of the coagulation mechanism in these patients. The increased absolute number of microparticles both total and platelet origin, also both positive and negative to annexin (MPs, PMPs, ANXNEG.MPs and ANXPOS.MPs) appeared to have affected the parameters of thromboelastometry in the patients of the present study, with apparent differences in CFT and MCF parameters that essentially express the differences in viscoelastic properties of patient blood clots compared to controls.
Main subject category:
Health Sciences
Keywords:
Thromboelastometry, Microparticles, Colon cancer, Hypercoagulation
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
124
Number of pages:
67
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