Estimation of the neurobiological parameters associated with depression in patients with chronic diseases

Postgraduate Thesis uoadl:2779503 1244 Read counter

Unit:
Κατεύθυνση Κλινική Βιοχημεία - Μοριακή Διαγνωστική
Library of the School of Science
Deposit date:
2018-08-02
Year:
2018
Author:
Megapanou Efstathia
Supervisors info:
Σταματάκης Αντώνιος , Αναπληρωτής Καθηγητής Βιολογίας- Βιολογίας Συμπεριφοράς, Τμήμα Νοσηλευτικής, ΕΚΠΑ
Original Title:
Εκτίμηση νευροβιολογικών παραμέτρων που σχετίζονται με κατάθλιψη σε άτομα με χρόνια σωματικά νοσήματα
Languages:
Greek
Translated title:
Estimation of the neurobiological parameters associated with depression in patients with chronic diseases
Summary:
Depression consists the most common psychological disorders impacting every aspect of a patient’s life. The disease belongs to a broader spectrum of disorders where the emotional disorder is the most eminent one. The illness is characterized by various and diverse clinical manifestations with the latent type to be the prevailing one. The etiology of the disease is multifactorial including genetic, family and social aspects while other causes such as traumatic or stressful events, serious or chronic illness and history of other mental disorders, play an important role, too. Treatment includes the combination of psychotherapy and antidepressant medications.
In this study, the correlation of the existence of depression in patients with Rheumatoid Arthritis and in individuals who do not suffer from a chronic illness was examined and it was attempted to determine the appearance of Major Depressive Disorder with the polymorphism BcII of the gene of the glucocorticoid receptor. The sample taken for this study is a part of the samples collected for the Research Programme “ASSERT-DEP” of the University of Ioannina which aims at studying the prevalence of Major Depressive Disorder (MDD) in patients with chronic diseases (Diabetes Mellitus, Rheumatological Diseases, Chronic Obstructive Pulmonary Disease) and the determination of the factors responsible for the resilience in MDD. 157 people participated with 55 of them having Rheumatoid Arthritis and 102 of them being in the control group. For the study, we extracted DNA from the participants’ peripheral blood and, then, using the methods of PCR, RFLP and electrophoresis in agarose gel, the polymorphism BCII (TGATCA→TGATGA) which is 646 nucleobases after the 3’ edge of the exon 2 of the gene of the glucocorticoid receptor (GR). The results showed that patients with Rheumatoid Arthritis -homozygotes or heterozygotes for the allele G- developed depression more frequently compared to the control group with the distribution of the genotypes in the public showing a statistically insignificant correlation with the development of the disease. In the future, the deeper understanding of the genetic factors which are involved in the development of certain endophenotypes will lead to the personalized and effective treatment.
Main subject category:
Science
Keywords:
Depression, Rheumatoid Arthritis, BcII, Gene of Glucocorticoid Receptor (GR)
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
45
Number of pages:
55
ΔΙΠΛΩΜΑΤΙΚΗ_ΜΕΓΑΠΑΝΟΥ ΕΥΣΤΑΘΙΑ_MSc ΚΛΙΝΙΚΗ ΒΙΟΧΗΜΕΙΑ.pdf (1 MB) Open in new window