Unit:
Κατεύθυνση Σακχαρώδης Διαβήτης και ΠαχυσαρκίαLibrary of the School of Health Sciences
Author:
Tzeravini Evangelia
Supervisors info:
Τεντολούρης Νικόλαος, Καθηγητής, Ιατρική Σχολή, Ε.Κ.Π.Α.
Μακρυλάκης Κωνσταντίνος, Αν. Καθηγητής, Ιατρική Σχολή. Ε.Κ.Π.Α.
Κόκκινος Αλέξαδνρος, Αν. Καθηγητής, Ιατρική Σχολή, Ε.Κ.Π.Α.
Original Title:
Εκτίμηση της μεταγευματικής λιπαιμίας στις δύο φάσεις του εμμηνορρυσιακού κύκλου μετά την κατανάλωση πρότυπου γεύματος
Translated title:
Assessment of the validity and reproducibility of a standarized test meal for the study of postprandial lipemia between the two mentrual cycle phases
Summary:
Aim: The aim of this intervational study was to evaluate the precision and reproducibility of examination using Lipotest® meal to assess postprandial lipaemia on two different occasions; luteal and follicular phase of menstrual cycle.
Material and Methods: Twenty five healthy, menstruating women participated in the trial. Each of them underwent an oral fat tolerance test (OFTT) twice; once during the follicular phase of menstrual cycle and once during the luteal phase. Prior to each visit participants had fasted overnight for 10-12 hours. Blood samples were collected at baseline (time 0, fasting) and 1, 2, 3, 4, 5 and 6 hours after consumption of the Lipotest® meal. Concentrations of triglycerides (TG) and apolipoprotein B (ApoB) were measured. The accuracy, precision and agreement of Lipotest® meal between the two phases of menstrual cycle were examined using Bland-Altman plots, correlation coefficient (r-value) and concordance correlation coefficient (CCC). Reproducibility was tested using the intraclass correlation coefficient (ICC). The area under the curve (AUC) of both TG and ApoB was also calculated.
Results: The precision and accuracy between the visits of luteal and follicular phase were moderate for TG values (r≤ 0.64, p<0.05, CCC<0.7,) and moderate to high for ApoB values(r > 0.7, p<0.001, CCC<0.7). The Bland-Altman plots depicted high agreement for both TG and ApoB values. Reproducibility of the measurements was good to excellent (ICC 0.689-0.848 for TG and 0.721-0.771 for ApoB). The values of the AUC between the two visits were not different for both TG and ApoB (p=0.114 and p=0.915 respectively)
Conclusion: This study has shown that an OFTT using Lipotest® meal for the assessment of postprandial lipemia among healthy, menstruating women has moderate accuracy, satisfactory precision and fair reproducibility of the TG measurements between the two menstrual phases; when ApoB values are taken into account, accuracy and precision are reinforced.
Main subject category:
Health Sciences
Keywords:
Postprandial lipemia, Triglycerides, Oral fat tolerance test, Menstrual cycle, Lipids, Accuracy, Reproducibility
Number of references:
313
