Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Author:
Kritikou Anastasia
Supervisors info:
Ευάγγελος Γκίκας, Επίκουρος Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Νικόλαος Θωμαΐδης, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Ιωάννης Ντότσικας, Επίκουρος Καθηγητής ΕΚΠΑ
Original Title:
Development of a multi-residue methodology for the determination of thyreostats in bovine muscle tissue by Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS)
Translated title:
Development of a multi-residue methodology for the determination of thyreostats in bovine muscle tissue by Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS)
Summary:
During the last decades, a large number of veterinary drugs have been used in order to improve animal health but also as growth promoters for intensive animal production. Antibiotics, anthelmintics and thyreostats are the most commonly used classes of drugs along with coccidiostats. Their improper use, non-respect of withdrawal periods, and cross-contamination can lead to the presence of veterinary drug residues in food of animal origin. The possible adverse effects on public health include allergic reactions in hypersensitive or sensitized individuals, and the development of resistant strains of bacteria following the ingestion of sub-therapeutic doses of antimicrobials.The possible presence of residues of veterinary drugs and other contaminants in edible tissues and food products is one of the main food safety issues of major concern to the public.
The aim of this study was the development of a sensitive, selective, resistant and effective analytical method for the determination of thyreostatic drugs 2-thiouracil (TU), 6-methyl-2-thiouracil (MTU), 6-propyl-2-thiouracil (PTU), 6-phenyl-2-thiouracil, methimazole (TAP), and 2-mercaptobenzimidazole (MBI) in food of animal origin using Hydrophilic Interaction Liquid Chromatography tandem Mass Spectrometry (HILIC-MS / MS).
Initially, an extended review of the veterinary drug classes and the existing methodology for the determination of thyreostatic drugs is presented. The experimental section of the thesis is constituted of two main parts: (1) Development and optimization of an analytical method for the determination of 6 thyreostatic drugs and (2) Method validation.
Main subject category:
Science
Keywords:
LC-MS/MS, HILIC, thyreostats, residue analysis, food of animal origin
Number of references:
172