Mechanisms of glucocorticoid actions: Investigating the in vivo role of hGRβ receptor in transgenic mice models

Postgraduate Thesis uoadl:2812505 313 Read counter

Unit:
Κατεύθυνση Εφαρμογές της Βιολογίας στην Ιατρική
Library of the School of Science
Deposit date:
2018-10-19
Year:
2018
Author:
Sigala Markezina
Supervisors info:
Ουρανία Τσιτσιλώνη, Αναπληρώτρια Καθηγήτρια Ανοσολογίας, Τομέας Φυσιολογίας Ζώων και Ανθρώπου, Τμήμα Βιολογίας, Πανεπιστήμιο Αθηνών
Παναγιώτα Παπαζαφείρη, Αναπληρώτρια Καθηγήτρια Φυσιολογίας Ζώων, Τομέας Φυσιολογίας Ζώων και Ανθρώπου, Τμήμα Βιολογίας, Πανεπιστήμιο Αθηνών
Πασχάλης Σιδεράς, Ερευνητής Α’, Κέντρο Κλινικής, Πειραματικής Χειρουργικής & Μεταφραστικής Έρευνας, Ίδρυμα ΙατροΒιολογικών Ερευνών Ακαδημίας Αθηνών
Original Title:
Μηχανισμοί δράσης των γλυκοκορτικοειδών: Διερεύνηση του ρόλου του υποδοχέα hGRβ in vivo σε διαγονιδιακά μοντέλα ποντικών
Languages:
English
Greek
Translated title:
Mechanisms of glucocorticoid actions: Investigating the in vivo role of hGRβ receptor in transgenic mice models
Summary:
Glucocorticoids (GCs) are hormones, essential for the stress response in humans and are commonly used in treating autoimmune, inflammatory and lymphoproliferative diseases. Their receptor (Glucocorticoid Receptor, GR) is an evolutionarily conserved protein, of the superfamily of nuclear receptors, regulating the diverse actions of glucocorticoids, acting as a transcription factor. This protein moves from the cytosol to the nucleus after binding to its ligand, GCs, and modifies the transcription rates of genes responding to glucocorticoids, in a positive or negative manner. There are many different isoforms of GR, one of which, GRβ, is very interesting, since it has a dominant negative effect on the functional GR isoform, GRα. The GRβ isoform is also correlated to pathologies or therapeutic consequences. In this study, we created transgenic mice that overexpress human GRβ, in order to determine the transcriptional levels of genes regulated by glucocorticoids and observe the endocrinological changes.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
glucocorticoids, receptor, stress, transgenic
Index:
Yes
Number of index pages:
4
Contains images:
Yes
Number of references:
38
Number of pages:
66
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