Supervisors info:
Χριστίνα-Άννα Μητσοπούλου, Καθηγήτρια, Χημείας, ΕΚΠΑ
Original Title:
Σύνθεση και χαρακτηρισμός των συμπλόκων [Re(CO)3Cl(dppz-3,6-COOΗ)] και [Re(CO)3Cl(dppz-3,6-COONa)]. Μελέτη της αλληλεπίδρασής τους με το DNA.
Translated title:
Synthesis and characterization of complexes [Re(CO)3Cl(dppz-3,6-COOΗ)] and [Re(CO)3Cl(dppz-3,6-COONa)]. DNA binding studies.
Summary:
In this thesis, is presented the synthesis of two novel Re(I) complexes, namely, [Re(CO)3Cl(dppz-3,6-COOΗ)] and [Re(CO)3Cl(dppz-3,6-COONa)], where dppz is bipyrido [3,2-a: 2 ', 3'-c] phenazine. These complexes have been synthesized and characterized by spectroscopic methods such as UV-visible absorption spectra, FT-IR, Electron Paramagnetic Resonance, Fluorescence and Cyclic Voltammetry.
Furthermore, it was studied their DNA binding (with Calf thymus DNA-C.T.-DNA) properties with a range of techniques such as UV-visible absorption spectra, Viscosity, Fluorescence, Cyclic Dichroism and Cyclic Voltammetry, while was calculated their Κb with DNA. All C.T.-DNA binding experiments were performed in Tris-HCl buffer at pH = 7. Finally, in vitro cytotoxicity studies of the complexes against MCF-7 and U87MG were determined using MTT assay.
Keywords:
Diimine complexes of Re (I), DNA binding, intercalation, cytotoxicity studies.
