Synthesis and Characterization of complex [Νi(qdt)(dppz)]. Study of its interaction with DNA and cancer cells MCF7 and MDA231

Postgraduate Thesis uoadl:2812909 431 Read counter

Unit:
Κατεύθυνση Ανόργανη Χημεία και Εφαρμογές της στη Βιομηχανία
Library of the School of Science
Deposit date:
2018-10-24
Year:
2018
Author:
Balou Sophie
Supervisors info:
ΧΡΙΣΤΙΝΑ – ΑΝΝΑ ΜΗΤΣΟΠΟΥΛΟΥ, Καθηγήτρια, τμήμα Χημείας, ΕΚΠΑ
Original Title:
ΣΥΝΘΕΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΟΥ ΣΥΜΠΛΟΚΟΥ [Νi(qdt)(dppz)]. ΜΕΛΕΤΗ ΤΗΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΤΟΥ ΜΕ ΤΟ DNA ΚΑΙ ΤΩΝ ΚΑΡΚΙΝΙΚΩΝ ΚΥΤΤΑΡΩΝ MCF7 ΚΑΙ MDA 231
Languages:
Greek
Translated title:
Synthesis and Characterization of complex [Νi(qdt)(dppz)]. Study of its interaction with DNA and cancer cells MCF7 and MDA231
Summary:
In this thesis, we present the synthesis of the complex [Ni(dppz)(qdt)], where the ligand dppz is the dipyrido [3,2-a:2',3'-c] phenazine and qdt is the ligand quinoxaline-2,3-dithiol. The aforementioned complex was characterized in the laboratory of Inorganic Chemistry by spectroscopic methods such as UV-vis spectroscopy, FT-IR spectroscopy, NMR spectroscopy and fluorescence. It was also characterized by cyclic voltammetry and Thermogravimetric analysis. Moreover, binding of the complex with Calf thymus DNA (C.T.-DNA) has been investigated by UV-visible absorption spectra, Viscosity analysis, Cyclic Voltammetry, Fluorescence and Cyclic Dichroism. DNA cleavage experiments have been also investigated by agarose gel electrophoresis using pBR322 DNA both in dark and after illumination with λ>400nm. Furthermore, MTT assay was used, aiming at the investigation of cell viability after incubation with the complex and the anticancer drug cis-platin.
Main subject category:
Science
Keywords:
Nickel complex, DNA binding, cell-toxicity
Index:
Yes
Number of index pages:
10
Contains images:
Yes
Number of references:
46
Number of pages:
117
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