Supervisors info:
ΧΡΙΣΤΙΝΑ – ΑΝΝΑ ΜΗΤΣΟΠΟΥΛΟΥ, Καθηγήτρια, τμήμα Χημείας, ΕΚΠΑ
Summary:
In this thesis, we present the synthesis of the complex [Ni(dppz)(qdt)], where the ligand dppz is the dipyrido [3,2-a:2',3'-c] phenazine and qdt is the ligand quinoxaline-2,3-dithiol. The aforementioned complex was characterized in the laboratory of Inorganic Chemistry by spectroscopic methods such as UV-vis spectroscopy, FT-IR spectroscopy, NMR spectroscopy and fluorescence. It was also characterized by cyclic voltammetry and Thermogravimetric analysis. Moreover, binding of the complex with Calf thymus DNA (C.T.-DNA) has been investigated by UV-visible absorption spectra, Viscosity analysis, Cyclic Voltammetry, Fluorescence and Cyclic Dichroism. DNA cleavage experiments have been also investigated by agarose gel electrophoresis using pBR322 DNA both in dark and after illumination with λ>400nm. Furthermore, MTT assay was used, aiming at the investigation of cell viability after incubation with the complex and the anticancer drug cis-platin.