Κατεύθυνση Χημική Ανάλυση-Έλεγχος Ποιότητας
Library of the School of Science

2018-11-05

2018

Papalekakos Pavlos

Α. Οικονόμου, Καθηγητής Χημείας, ΕΚΠΑ
Ν. Θωμαΐδης, Αναπλ. Καθηγητής Χημείας, ΕΚΠΑ
Ι. Ντότσικας, Επικ. Καθηγητής Φαρμακευτικής, ΕΚΠΑ

Ανάπτυξη και Επικύρωση Μεθόδου Ταυτοποίησης Τζινσενοσιδών σε Συμπληρώματα Διατροφής με χρήση Υγροχρωματογραφίας - Φασματομετρίας Μαζών

Greek

Development and Validation of a method for determination of Ginsenosides by Liquid Chromatography - Mass Spectrometry

The aim of this study is to develop and validate a method for the simultaneous determination of four of the active ingredients which are found in ginseng. These chemical compounds are called ginsenosides and they are responsible for the beneficial properties of ginseng as a food supplement. The identification of each ginsenoside was determined in a dry extract by performing screening experiments with a system performing liquid chromatography (LC) and mass spectrometry (MS). Subsequently this method was transfered and developed to a system of high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA). During the chromatographic separation, we used an analytical C18 column and gradient elution with a mobile phase of water- acetonitrile mixture which had previously been acidified to pH = 2 with . After the validation of this method, it was used to determine and identify the ginsenosides that are contained in food supplements.

Science

Ginseng, Ginsenosides, Diodearray detector, Validation, Korean Ginseng, Panax Ginseng

Yes

4

Yes

69

137

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https://pergamos.lib.uoa.gr/uoa/dl/object/2817639