Τομέας Κλινικοεργαστηριακός
Library of the School of Health Sciences

2018-11-07

2018

Maragkoudaki Maria

Μαρία Γαζούλη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Θεοδωρόπουλος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Ζωγράφος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μαρία Κυριακοπούλου – Λυμπέρη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ευάγγελος Μαρίνος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Καραμανώλης, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Σπήλιος Μανωλακόπουλος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Μελέτη της έκφρασης των δεικτών πολυδυναμικότητας στις ιδιοπαθείς φλεγμονώδεις νόσους του εντέρου

Greek

Specific detection of OCT4 isoforms in inflammatory bowel disease

Developmentally early cells are mobilized into peripheral blood in Crohn's disease (CD) patients. OCT4, is considered to be important in sustaining the pluripotency of stem cells. OCT4 splicing variants are differentially expressed in pluripotent and non-pluripotent cells. Our study aims to investigate the expression pattern of OCT4 variants and SOX-2, an essential factor implicated in self-renewal and pluripotency, in tissue and blood samples from patients with IBD.
Methods: Peripheral blood and tissue samples were collected from patients with active CD and ulcerative colitis (UC), and from healthy individuals. OCT4 expression was documented by Western blot, immunohistochemistry and by reverse transcription-real-time PCR. OCT4 isoform determination was documented using specific primers. SOX-2 expression levels were also evaluated.
Results: OCT4 protein levels were significantly higher in CD tissue samples than in CD blood samples, and in UC tissue samples. OCT4 protein was localized mainly in the cytosol. In all samples, only the OCT4 pseudogenes and the OCT4B1 variant were detected. OCT4B1 expression levels were elevated in both tissue and blood samples from CD and UC cases compared to healthy controls. In CD patients only SOX-2 mRNA levels were found slightly increased compared to healthy controls.
Conclusion: Our results suggest that OCT4 is expressed in patients with IBD. Furthermore, we found the presence of the OCT4B1 isoform in IBD in both tissue and blood samples. Our results have shown, that developmentally early cells might be mobilized into peripheral blood as result of tissue damage, indicating a possible role of these cells in repair of injured intestinaltract.

Health Sciences

IBD, Crohn’s disease, Ulcerative colitis, Stem cells, Mobilization

No

0

Yes

300

136

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https://pergamos.lib.uoa.gr/uoa/dl/object/2818838