Correlation of physical fitness, measured in middle age, with body resistance to stress in old age at Drosophila

Postgraduate Thesis uoadl:2820400 355 Read counter

Unit:
Κατεύθυνση Μοριακή και Εφαρμοσμένη Φυσιολογία
Library of the School of Health Sciences
Deposit date:
2018-11-25
Year:
2018
Author:
Papadopoulou Marina
Supervisors info:
Χρήστος Κόνσουλας, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μιχαήλ Κουτσιλιέρης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Αναστάσιος Φιλίππου, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Συσχετισμός της φυσικής κατάστασης, μετρούμενης στη μέση ηλικία, με την αντοχή του οργανισμού στο stress κατά την τρίτη ηλικία στη Drosophila
Languages:
Greek
Translated title:
Correlation of physical fitness, measured in middle age, with body resistance to stress in old age at Drosophila
Summary:
OBJECTIVE: This study aimed to study the relationship between state of physical status and lifespan under stressful conditions in the experimental model Drosophila Melanogaster (type: Oregon-R).
METHODS: Flies 40-60 days old were anesthetized with carbon dioxide to isolate the males. The climbing speed of every individual fly was measured by geotaxis assay with a Conrad camera 24 hours later. The same procedure was carried out in all 3 experiments on desiccation, starvation and oxidative stress.
RESULTS: In desiccation and starvation stress lifespan was not correlated with the physical condition of the animals. However, in oxidative stress, it was observed that flies that had a longer lifespan were in a better physical condition.
CONCLUSIONS: Thus, under oxidative stress, physical fitness predicts resistance to stress. Faster flies lived longer. In contrast, the very intense desiccation and starvation stresses did not allow this correlation to evolve.
Main subject category:
Health Sciences
Keywords:
Physical fitness, Stress, Drosophila melanogaster, Lifespan, Aging
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
173
Number of pages:
69
Papadopoulou Marina Master.pdf (1 MB) Open in new window