Unit:
Κατεύθυνση Διεθνής Ιατρική - Διαχείριση Κρίσεων ΥγείαςLibrary of the School of Health Sciences
Supervisors info:
Κοντός Μιχαήλ, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Γεωργόπουλος Σωτήριος, Καθηγητής, Ιατρική, ΕΚΠΑ
Καραβοκυρός Ιωάννης, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Μελέτη του ρόλου της κινάσης Aurora στo παράσιτo Leishmania spp., μέσω διαγονιδιακών στελεχών του παρασίτου
Translated title:
Investigating the role of Aurora kinase in the parasite Leishmania spp., using transgenic strains of the parasite
Summary:
Leishmaniasis is a potentially fatal disease of major health significance globally. On the road of discovering new antileishmanial drugs, we followed the Structure Based Drug Design strategy (SBDD), which relies on the development of new drugs concerning the structure of molecular targets. One prominent drug target for other diseases including cancer, is Aurora kinase, a protein involved in cellular division of eukaryotic cells, a process which results in genetic instability when is dysregulated. Previous work in the laboratory of Molecular Parasitology (MP) unit of Hellenic Institute Pasteur (HIP) have shown that parasite’s kinase Aurora (LinAuroraK) is essential for parasite’s viability. Subsequently mutants or wild-type forms of LinAuroraK were overexpressed in L. infantum transgenic strains, anticipating that mutant kinases will have a dominant negative effect on wild-type LinAuroraK. Main goal of the present research is to study the effect of these mutations on the growth rate, cell death, cell cycle functions and phenotype of the transgenic strains, in order to investigate the role of LinAuroraK in Leishmania parasites. Our results demonstrate the importance of LinAuroraK on cell cycle process, mainly by involving on the G2M phase and the importance of residues in the predicted active site (K60), sumoylation site (K156) or activation loop (T186), for kinase’s function.
Main subject category:
Health Sciences
Keywords:
Leishmania, Trypanosomatids, Aurora kinases, Drug targets, Molecular parasitology
Number of references:
132
File:
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ΓΛΥΜΗΣ ΔΕΔΟΥΣΗΣ ΔΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ.pdf
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