An experimental study of the regional and systematic effect of Lactobacilli species in short bowel syndrome in rats

Postgraduate Thesis uoadl:2837841 447 Read counter

Κατεύθυνση Βιοστατιστική
Library of the School of Health Sciences
Deposit date:
Stoidis Christos
Supervisors info:
Αναστάσιος Μαχαίρας, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ελένη-Κλεάνθη Κατσουγιάννη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Φώτιος Σιάννης, Επίκουρος Καθηγητής, Τμήμα Μαθηματικών, ΕΚΠΑ
Original Title:
Πειραματική μελέτη της περιοχικής και συστηματικής επίδρασης ειδών λακτοβάκιλλων στο σύνδρομο του βραχέος εντέρου σε επίμυες.
Translated title:
An experimental study of the regional and systematic effect of Lactobacilli species in short bowel syndrome in rats
Background and Purpose: The effect of symbiotics (SYMs) after massive small bowel resection on a cellular intestinal and especially extra- intestinal level has not been established yet. With our novel experimental study we evaluated the effect of SYMs on the phenomena of apoptosis and lipopolysaccharide (LPS) endotoxinemia.
Materials and Methods: In this two- week study we used male Wistar rats that were divided into seven groups (n= 10/group): a control group (non operated/ fed regular diet), a sham group (bowel transection and re- anastomosis/ fed regular diet), four experimental groups (75% small bowel resection/ ± symbiotic intake pre- or post OP) and a final group of non- operated animals fed with SYMs enriched diet. Intestinal structural changes, apoptosis (intestinal, liver, splenic, pulmonary and cardiac cells), LPS serum levels and bacterial translocation (BT) of gram negative intestinal bacteria to mesenteric lymph nodes, liver, spleen, and peripheral blood were determined.
Results: Treatment with SYMs resulted in an increase in villus height (905.236±54.892 vs 787.154±17.523 μm, p<0.05) and in a decrease in BT (80% vs 100%, p>0.05), apoptotic index (110.188±2.824 vs 99.302±3.284, p<0.05), lymphocyte mucosal infiltration (30% vs 50%, p>0.05) and LPS serum levels (1.67±0.322 vs 3.831±1.544 eu/ml, p<0.05) compared with untreated animals.
Conclusions: In a rat model SYMs seem to enhance the intestinal barrier and protect the cellular structure from the local and systematic effects of SBS.
Main subject category:
Health Sciences
Symbiotics; Short bowel syndrome; Rat model; Bacterial translocation; Apoptosis; Intestinal mucosa.
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