Ε. Ι. Γιαμαρέλλος-Μπουρμπούλης, Καθηγητής, Ιατρική, ΕΚΠΑ
Σ. Καλανταρίδου, Καθηγήτρια, Ιατρική, ΕΚΠΑ
Χ. Γ. Χρέλιας, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Α. Αντωνιάδου, Αναπληρώτρια Καθηγήτρια, Ιατρική, ΕΚΠΑ
Χ. Συριστατίδης, Αναπληρωτής Καθηγήτης, Ιατρική, ΕΚΠΑ
Α. Παπαδόπουλος, Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Π. Παναγόπουλος, Επίκουρος Καθηγητής, Ιατρική, ΕΚΠΑ
Problem To study the balance of circulating Heat shock protein Hsp60 and Hsp70 in preterm delivery
Method of study A two-stage approach was used. At first stage we run retrospective analysis of prospective collected clinical data and at a second stage we studied an animal model of preterm delivery (PTD). Blood samples were collected for prenatal screening in 3,629 women. Samples from 23 women with miscarriage before gestational week 21 and 53 well-matched comparators for age, body mass index, parity and previous miscarriage with full-term pregnancy were depicted. Women with risk factors were excluded. Hsp60 and Hsp70 were measured by an enzyme immunosorbent assay. PTD was induced after injection of low dose of bacterial lipopolysaccharide; mice were sacrificed for the measurement of Hsp60 and Hsp70 in blood and tissues. The study endpoint was the association of the Hsp60 to Hsp70 ratio to miscarriage.
Results A ratio greater than 6 could distinguish between women who will miscarry from women with term pregnancies with sensitivity 60%, specificity 81.8%, positive predictive value 81.8% and negative predictive value 60% (OR: 6.750, p= 0.025). Mice of the LPS-group PTD had this ratio significantly increased in maternal serum, placentas and embryos compared to the sham-operation group. Gene expression of Hsp60/70 remained in tissues unaltered.
Conclusions An Hsp60/Hsp70 ratio equal to or more than 6 until gestational week 12 is accompanied with great likelihood for miscarriage. A similar ratio applies in an animal model of PTD induced by low-dose LPS.