Unit:
Κατεύθυνση Ιατρική Γενετική: Κλινική και Εργαστηριακή ΚατεύθυνσηLibrary of the School of Health Sciences
Supervisors info:
Τζέτη Μαρία, Αναπληρώτρια Καθηγήτρια, Ιατρική, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Φρυσίρα Ελένη, Καθηγήτρια, Ιατρική, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Traeger-Συνοδινού Ιωάννα-Ραχήλ, Καθηγήτρια, Ιατρική, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Μελέτη της επίδρασης εξωγενώς χορηγούμενης ερυθροποιητίνης (EPO) στην αγγειογένεση μετά από πρόκληση ισχαιμίας σκέλους σε μύες
Translated title:
Study of the effect of exogenously administered erythropoietin (EPO) on angiogenesis after causing artificial limb ischemia on mice
Summary:
This thesis deals with angiogenesis generated after causing artificial limb ischemia on mice. Laser-Doppler imaging and Real-time PCR were carried out to observe the progress of blood circulation on both lower extremities, after administrating either erythropoietin or saline. In addition, reference is made to the theoretical background of critical limb ischemia, as well as peripheral arteriopathy.
The aim of this study is to present the effect of erythropoietin administration on mice in leg-induced limb ischemia and the potential use of this method as a treatment on patients with similar clinical situation.
Male WTC57BL/6 mice 10 weeks old were used for this study. After anesthesia, the left femoral artery was ligated and removed (ischemia). During the 5 days following the surgical procedure, 0,2 mL EPO (400 IU / kg) were administered to the EPO group and 0,2 mL saline were administered to the control group. Blood flow was evaluated by Laser-Doppler imaging on the 1st, 2nd and 3rd day after ischemia. The mice underwent euthanasia on the 28th day of the experiment and both of their legs’ quadriceps muscles were removed and stored in liquid nitrogen. Total RNA was isolated from the tissue samples, and cDNA was synthesized and the expression of angiopoietin-1 was studied by Real-time PCR.
The results from Laser-Doppler imaging showed similar values in both groups: no change on day 1, an increase on day 7 and decrease on day 28. The change in angiopoietin-1 expression by Real-time PCR is not statistically significant for the EPO group compared to the control group.
Main subject category:
Health Sciences
Keywords:
Erythropoietin, Angiopoietin-1, Angiogenesis
