Development and validation of a method for the determination of polycyclic aromatic hydrocarbons in samples of follicular fluid

Postgraduate Thesis uoadl:2865782 220 Read counter

Unit:
Κατεύθυνση Αναλυτική Χημεία
Library of the School of Science
Deposit date:
2019-03-12
Year:
2019
Author:
Maravelia Eleftheria
Supervisors info:
Μπακέας Ευάγγελος, Αναπληρωτής Καθηγητής, τμήμα χημείας, ΕΚΠΑ
Οικονόμου Αναστάσιος, Καθηγητής ,τμήμα χημείας, ΕΚΠΑ
Κόκκινος Χρήστος, Επίκουρος Καθηγητής ,τμήμα χημείας, ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδου προσδιορισμού πολυκυκλικών αρωματικών υδρογονανθράκων σε δείγματα ωοθυλακικού υγρού
Languages:
English
Greek
Translated title:
Development and validation of a method for the determination of polycyclic aromatic hydrocarbons in samples of follicular fluid
Summary:
The current thesis presents the development and validation of a method for the determination of Polycyclic Aromatic Hydrocarbons in follicular fluid, using gas chromatography mass spectrometry, as well as the attempt of correlating environmental factors as markers related to infertility
For this purpose, a sufficient number of follicular fluid samples, of women undergoing in vitro fertilization, were collected. The full validation of the method includes precision tests, repeatability and reproducibility tests, recovery tests and LoD and LoQ calculation.
The method approved to be suitable for the PAHs measurement in real samples. PAHs were measurable nearly in all samples of women undergoing in vitro fertilization.
The samples originated from Greece, but mainly from Attica region. Also demographic data, such as age, place of residence etc, were collected, which could help the processing of the results and conclusions.
Main subject category:
Science
Keywords:
Polycyclic aromatic hydrocarbons, organic pollutants, gas chromatography, mass spectrometer, follicular fluid, infertility, IVF
Index:
Yes
Number of index pages:
2
Contains images:
Yes
Number of references:
97
Number of pages:
88
ΑΝΑΠΤΥΞΗ ΚΑΙ ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΟΥ ΠΡΟΣΔΙΟΡΙΣΜΟΥ ΠΟΛΥΚΥΚΛΙΚΩΝ ΑΡΩΜΑΤΙΚΩΝ ΥΔΡΟΓΟΝΑΝΘΡΑΚΩΝ ΣΕ ΔΕΙΓΜΑΤΑ ΩΟΘΥΛΑΚΙΚΟΥ ΥΓΡΟΥ.pdf (1 MB) Open in new window