Determination of Bisphenol A in food simulants IN FOOD SIMULANTS A,B,C AND D1 PERFORMED BY HPLC-FLD AND VALIDATION OF THE METHOD

Postgraduate Thesis uoadl:2877121 295 Read counter

Unit:
Κατεύθυνση Χημεία Τροφίμων
Library of the School of Science
Deposit date:
2019-06-28
Year:
2019
Author:
Nana Konstantina
Supervisors info:
Χαράλαμπος Προεστός
Επίκουρος Καθηγητής Χημείας Τροφίμων,
Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Μελέτη μετανάστευσης χημικών ουσιών από πλαστικά υλικά και αντικείμενα που προορίζονται να έρθουν σε επαφή με τρόφιμα μετά τον προσδιορισμό ολικής και ειδικής μετανάστευσης Δισφαινόλης Α
Languages:
Greek
Translated title:
Determination of Bisphenol A in food simulants IN FOOD SIMULANTS A,B,C AND D1 PERFORMED BY HPLC-FLD AND VALIDATION OF THE METHOD
Summary:
Bisphenol A, an endocrine disruptor, is the monomer used to make polycarbonate plastics (PC) so it is widely found in food packaging materials. Complementary to the Regulation (EU) 10/2011, European Commission adopted the Regulation (EU) 2018/213 prohibiting migration of BPA found in varnishes and coatings intended to come into contact with infant food. The aim of this work was to develop and validate a fit for purpose method for the determination of BPA following overall migration in aqueous food simulants (A, B, C and D1) from plastic materials and articles intended to come into contact with food. Determination of total migration was carried out by article filling with the corresponding simulator and contact temperature and time conditions at 2 hours at 70oC. Qualitative and quantitative determination of BPA was performed by a High Performance Liquid Chromatographer (HPLC) coupled to a Fluorometric Detector (FLD). The analysis resulted in fast elution time of BPA (around 4 minutes) by using a C18 column applied. The method was validated; LODs and LOQs varied from 0.42 to 2.12 μg L-1 and 1.31 to 6.43 μg L-1 respectively. Recovery, precision, trueness, linearity, uncertainties, repeatability, reproducibility fulfilled all the requirements set by ISO 17025. Trueness of the method developed, regarding A and B simulators, was proved by the successful interlaboratory test (Proficiency test) that we participated in (z-scores in simulants A and B were 0.60 and 0.33 respectively). This research resulted in a rapid, cost effective and environmental friendly green method for BPA specific determination.
Main subject category:
Science
Keywords:
Bisphenol A, BPA, food contact materials, food simulants, specific migration
Index:
Yes
Number of index pages:
4
Contains images:
Yes
Number of references:
33
Number of pages:
119
File:
File access is restricted only to the intranet of UoA.

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