Study of the effect of aging on phenolic compounds and the antioxidant activity of red wines, using spectrophotometric methods.

Postgraduate Thesis uoadl:2877582 210 Read counter

Unit:
Κατεύθυνση Βιομηχανική Χημεία
Library of the School of Science
Deposit date:
2019-07-03
Year:
2019
Author:
Papadimitriou Panagiotis-Konstantinos
Supervisors info:
Λιούνη Μαρία, Αναπληρώτρια Καθηγήτρια ΕΚΠΑ
Παπαδογιαννάκης Γεώργιος,Καθηγητής ΕΚΠΑ
Δέτση Αναστασία, Αναπληρώτρια Καθηγήτρια ΕΜΠ
Original Title:
Μελέτη επίδρασης της παλαίωσης στις φαινολικές ενώσεις και στην αντιοξειδωτική δράση ερυθρών οίνων, με χρήση φασματοφωτομετρικών μεθόδων.
Languages:
Greek
Translated title:
Study of the effect of aging on phenolic compounds and the antioxidant activity of red wines, using spectrophotometric methods.
Summary:
This dissertation deals with the influence of aging on the total phenolic content
and the antioxidant capacity in red dry wines from the region of Nemea,
Korinthia and the prefecture of Heraklion, Crete, as well as the calculation of
these values using spectrophotometric methods.
Wines and especially red wines contain phenolic compounds, which have a
great biological role for the human organism and because of their important
properties such as antioxidant action, they help to prevent various diseases.
The spectrophotometric methods used were: Folin-Ciocalteu for the
calculation of total phenolic content and FRAP, CUPRAC, DPPH and ABTS
for the determination of antioxidant capacity.
Initial dilutions are made in the wine samples, then the reagents of each
method are added and, after stirring and incubating under suitable conditions
and time to perform the chemical reactions, the absorbance is recorded on an
uv spectrophotometer. At the same time a standard curve of a standard
phenolic compound is formed and the results expressed as equivalents of this
substance.
Finally, because the above methods differ from one another in terms of
analysis principles and reaction conditions, only one method is not enough to
extract a safe balance about antioxidant activity. Thus, this dissertation
compared the results of four widely used spectrophotometric methods.
Main subject category:
Science
Keywords:
Antioxidant action, Aging, DPPH, ABTS, FRAP.
Index:
Yes
Number of index pages:
8
Contains images:
Yes
Number of references:
162
Number of pages:
149
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