Development and validation of methods for the determination of polar pesticide residues in rice by liquid chromatography-tandem mass spectrometry

Postgraduate Thesis uoadl:2877901 202 Read counter

Unit:
Κατεύθυνση Αναλυτική Χημεία
Library of the School of Science
Deposit date:
2019-07-05
Year:
2019
Author:
Thoma Niki-Aimilia
Supervisors info:
Αναστάσιος Οικονόμου, Καθηγητής ΕΚΠΑ Τμήμα Χημείας
Original Title:
Ανάπτυξη και επικύρωση μεθόδων προσδιορισμού υπολειμμάτων πολικών γεωργικών φαρμάκων σε ρύζι με υγροχρωματογραφία-φασματομετρία μαζών σε σειρά
Languages:
Greek
Translated title:
Development and validation of methods for the determination of polar pesticide residues in rice by liquid chromatography-tandem mass spectrometry
Summary:
In this work two single-residue methods for the determination of 6 polar pesticide residues, chlormequat, mepiquat, paraquat, glyphosate, glufosinate, ethephon, in rice were developed and validated. The sample preparation was performed using the QuPPE method (Quick Polar Pesticides Extraction) and the extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the detection and quantification of 6 polar pesticides mentioned above. The method was validated according to the EU Guidelines for pesticide residues SANTE/11813/2017 for linearity, accuracy and precision (repeatability and reproducibility) while the limit of detection and quantification were evaluated along with the matrix effect.
The detection and the quantification of the analytes was performed with LC-MS/MS with electro-spray ionisation (LC-ESI-MS/MS). The analytical method proved fit-for-purpose for the determination of the six polar pesticides in rice samples, as it has satisfactory analytical performance and is also simple, rapid and cost-effective.
Main subject category:
Science
Keywords:
polar pesticides, rice, liquid chromatography, mass spectrometry, validation, QuPPE
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
76
Number of pages:
105
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