Supervisors info:
Ράλλης Μιχαήλ, Επίκουρος Καθηγητής, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Συγκριτική in vivo μελέτη αποτελεσματικότητας τριών τοπικών σκευασμάτων σε μοντέλο ατοπικής δερματίτιδας άτριχων μυών & Κλινική μελέτη του δραστικότερου τοπικού σκευάσματος με πρόκληση οξείας ερεθιστικής δερματίτιδας σε άτομα με ιστορικό ατοπίας και υγιείς μάρτυρες με ταυτόχρονη αξιολόγηση της αποκατάστασης του επιδερμικού φραγμού
Translated title:
Comparative in vivo study of the efficacy of three topical preparations in a hairless mouse model of atopic dermatitis & Clinical study of the most active topical preparation by inducing acute irritant dermatitis in people with a history of atopy and healthy controls with simultaneous assessment of epidermal barrier repair
Summary:
Atopic dermatitis is a chronic, recurrent, inflammatory and highly pruritic skin disease. It is one of the most common skin disorders, affecting approximately 25% of children and 2-3% of adults. This disease has a complicated pathogenesis, including genetic, immunological and environmental factors, leading to impairment of the epidermal barrier and the immune system. It also presents a variety of clinical features (redness, oedema, dry skin, papillary or exudative lesions, lechenification) that are highly affected by the age of the patient. From the above, it is clear that atopic dermatitis is a major health problem, affecting the quality of life of the patients and public health.
In the present survey, a comparative in vivo study of the activity of three topical preparations was conducted in a hapten-induced atopic dermatitis mouse model. Atopic dermatitis was induced by epidermal applications of the hapten DNCB (2,4-dinitrochlorobenzene), and this method presents similar histopathological, immunological and clinical features compared to human atopic dermatitis.
The following topical preparations were tested:
i. ointment containing Ceratothoa oestroides Oil Extract (10%)
ii. ointment containing Ceratothoa oestroides Oil Extract (10%) and Chlorocresol (0,1%)
iii. ointment containing Ceratothoa oestroides Oil Extract (10%), Chlorocresol (0,1%) and Olea Europaea Oil Extract, containing the active ingredients oleicanthal and oleasin (2:1 ratio)
The local preparations were applied throughout the dorsal area of the mice (dose 5mg/cm2), once daily for 20 days. The analysis of the results showed that the ointment containing Ceratothoa oestroides Oil Extract, Chlorocresol and Olea Europaea Oil Extract (oleicanthal and oleasin) had the best results, as histopathologically showed almost completely healthy skin regions and the best cinical features compared to the other treatment groups.
A clinical study was then conducted in the Patch Testing Unit of Andreas Syggros Hospital of Cutaneous & Venereal Diseases, in accordance with the principles of good clinical practice of the Helsinki Declaration. The purpose of this survey was to examine the efficacy and safety of the superior topical preparation (ointment containing Ceratothoa oestroides Oil Extract, Chlorocresol and Olea Europaea Oil Extract) in order to study the repair of impaired epidermal barrier function. The epidermal barrier was impaired by induced acute irritant dermatitis (controlled reaction), in patients with a history of atopy and in healthy controls. Acute irritant dermatitis was induced by applying 4% Sodium Lauryl Sulfate (SLS) solution for 24 hours in the volunteer’s arm. The efficacy of the test topical preparation was compared with a commercial formulation which has proven efficacy. The study is underway, so no results and conclusions can be drawn.
Keywords:
atopic dermatitis, topical therapy, hairless mouse model of atopic dermatitis, Ceratothoa oestroides extract, chlorocresol, Olea Europaea Oil Extract (oleicanthal and oleasin)
