Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Author:
Trigonis Georgios-eleftherios
Supervisors info:
Θωμαΐδης Νικόλαος, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Οικονόμου Αναστάσιος, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Γκίκας Ευάγγελος, Επίκουρος Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Original Title:
ΑΝΑΠΤΥΞΗ ΚΑΙ ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΟΥ HPLC ΚΑΙ LC-MS/MS ΓΙΑ ΤΟΝ ΠΟΣΟΤΙΚΟ ΠΡΟΣΔΙΟΡΙΣΜΟ ΤΩΝ ΦΑΡΜΑΚΟΥΧΩΝ ΕΝΩΣΕΩΝ, ΦΛΟΥΜΕΚΙΝΗς ΚΑΙ ΕΜΑΜΕΚΤΙΝΗΣ, ΣΕ ΣΚΕΥΑΣΜΑΤΑ ΙΧΘΥΟΤΡΟΦΩΝ
Translated title:
DEVELOPMENT AND VALIDATION OF METHOD HPLC AND LC-MS/MS FOR THE QUANTITATIVE DETERMINATION OF PHARMACEUTICAL COMPOUNDS FLUMEQUINE AND EMAMECTINE ON FISH FEED
Summary:
The purpose of the present study was to develop a rapid Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method for quantification of flumequine in fish feed formulations and a rapid LC-ESI-MS / MS method ( Liquid Chromatography-Electrospray Ionization Mass Spectrometry Mass Spectrometry) for the detection and quantification of emamectin in fish feeds formulations. Their detection and quantification were carried out using two C18 columns and an extensive series of experiments was performed to improve experimental and chromatographic parameters (pH, elution time, traceability and extractability). During the development and validation of the methods for both flumequine and emamectin were carried out the following experiments such as linearity, accuracy, precision, robustness and experiments for the determination of quantification limits and detection limits (LOQ and LOD) respectively.
Main subject category:
Science
Keywords:
Pharmaceutical compounds, HPLC, LC-MS / MS, validation, traceability
