Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Author:
Anastasopoulos Ioannis
Supervisors info:
Νικόλαος Θωμαΐδης, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδων για τον προσδιορισμό φαρμακούχων ενώσεων σε ιχθυοτροφές, με χρήση τεχνικών υγροχρωματογραφίας με ανιχνευτές ορατού υπεριώδους (UV-VIS), φωτοδιόδων (DAD) και διαδοχικής φασματομετρίας μαζών (MS/MS)
Translated title:
Development and validation of analytical methods for the determination of medicated compounds in fish feed using HPLC with UV-Visible Detector, Photodiode Array Detector and sequential mass spectrometry (MS / MS)
Summary:
In this thesis, we study the development and validation of analytical methods, for the determination of medicated compounds in fish feeds. Specifically, it relates to the determination of the compounds: Oxytetracycline Hydrochloride, Sulphadiazine and Trimethoprim. The aim is to determine the content of raw materials used for the production of fish feed, the final product and the cleaning line. For the development of the above methods, the high-performance liquid chromatography technique was used with a visible / ultraviolet detector (HPLC UV / VIS) and diode array detector (DAD). To determine the trimethoprim in the purification lines, the liquid chromatography coupled with mass spectrometry (LC MS / MS) was used, because of the very low concentrations. The experimental part is divided into two axes. Initially develop and optimize the above methods and then validate them.
As a result, for oxytetracycline hydrochloride, a method that allows its determination in the raw material, the final product as well as the purification line has been validated.
Similarly, for the other two compounds, a common method for determining the raw material and the final product was validated. As regards the purification line for sulfadiazine, the above method was used, while for the trimethoprim another method was validated in LC MS / MS.
Main subject category:
Science
Keywords:
Oxytetracycline hydrochloride, Sulphadiazine, Trimethoprim, HPLC UV/VIS – DAD, LC MS/MS
