Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Supervisors info:
Αναστάσιος Οικονόμου,Καθηγητής, Τμήμα Χημείας, Ε.Κ.Π.Α.
Σωτήριος Κακαμπάκος, Ερευνητής Α΄, Ε.ΚΕ.Φ.Ε. «ΔΗΜΟΚΡΙΤΟΣ»
Χρήστος Κόκκινος, Επίκουρος Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
«ΑΝΑΠΤΥΞΗ ΕΝΖΥΜΟΑΝΟΣΟΠΡΟΣΔΙΟΡΙΣΜΩΝ ΜΕ ΧΡΗΣΗ ΠΟΛΥΚΛΩΝΙΚΟΥ ΑΝΤΙΣΩΜΑΤΟΣ ΕΝΑΝΤΙ ΤΗΣ ΒΟΕΪΟΥ κ-ΚΑΖΕΪΝΗΣ ΓΙΑ ΤΗΝ ΑΝΙΧΝΕΥΣΗ ΝΟΘΕΙΑΣ ΑΙΓΟΠΡΟΒΕΙΟΥ ΓΑΛΑΚΤΟΣ ΚΑΙ ΤΥΡΙΟΥ ΜΕ ΑΓΕΛΑΔΙΝΟ»
Translated title:
Development of a competitive ELISA method for the detection of goat/ewe's milk and cheese adulteration with cow's milk.
Summary:
Adulteration of goat/sheep or sheep milk with use of cow's milk is often observed in the trade of dairy products and affects a huge part of the commercial community as well as vulnerable groups of consumers that present allergy in specific diary products. Because of that, the global organisations of quality assurance have established rules and thresholds that force food industries to declare both the origin of diary products and their ingredients, in order to protect the financial rights of consumers as well as to reduce the danger that public health is exposed to. In order to avoid adulteration, sample testing is mandatory in diary industries so as to detect possible mixtures with milk of different origin or milk serum. The objective of this diploma thesis is the development of an enzymatic immunochemical competitive type method for the detection of bovine k-casein in samples of goat/sheep milk and cheese with the use of a specific polyclonal antibody against bovine k-casein, produced in our lab. The method was assayed as to its analytical characteristics and the ability to detect adulteration of goat/sheep milk and cheese with bovine. In order to accomplish this task, all the parameters of this method were tested and optimised using as criteria the intensity of the analytical signal, the detection sensitivity, the work place, the accuracy, the inter- and intra-analytical repeatability, the specificity and the duration of the analysis. The method which was developed is quick, sensitive and accurate with detection limits comparable or even lower than that of commercially available methods being used by industry. The detection limits of this method for both milk and cheese were lower than 1%, a threshold set by EU legislation for the control of adulteration and mixture rate. As a result, this method can be used as a reliable method of quality assurance by diary production industries for testing and detecting adulteration of goat milk and cheese by cow's milk.
Main subject category:
Science
Keywords:
adulteration, milk, cheese, ELISA, k-casein
