Unit:
Κατεύθυνση Κοσμητολογία - ΔερματοφαρμακολογίαLibrary of the School of Science
Supervisors info:
Ρούσσης Βασίλειος, Αναπληρωτής Καθηγητής, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας
Original Title:
Αξιολόγηση της επουλωτικής δράσης επιλεγμένων εκχυλισμάτων και συστατικών από το θαλάσσιο ισόποδο Ceratothoa oestroides
Translated title:
Evaluation of the healing activity of selected extracts and constituents from the sea isopod Ceratothoa oestroides
Summary:
Ceratothoa oestroides is a protandric hermaphrodite parasite of the order Isopoda, infesting a wide range of fish species. The aim of the present study was to evaluate the activity of different extracts of the isopod in the healing process, as well as to find their optimal dose. The organic extract of the isopod was subjected to liquid-liquid extraction with solvents of different polarity to afford four fractions. Those four fractions were then formulated in ointments and their wound healing activity was evaluated on the normal skin of hairless mice type SKH-hr1, after excision of an 1 cm2 of the upper back skin area. The most significant healing effect was observed for the dichloromethane fraction, which was enriched in polyunsaturated fatty acids (PUFA).
The histopathological analysis of the skin of mice treated with the dichloromethane fraction, showed mild to minimal inflammation, accelerating tissue regeneration. Since the particular fraction contained a high concentration of polyunsaturated fatty acids (PUFA), the activity of one component (EPA) of the particular extract was then individually tested, to check if the healing effect was due to this constituent and whether the effect is synergistic.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
healing, activity, isopod, Ceratothoa, oestroides, fraction, extract, mice
Number of references:
126
