Immunohistochemical study of early dental pulp responses after direct pulp capping in an ex vivo human tooth model

Postgraduate Thesis uoadl:2879010 247 Read counter

Unit:
Κατεύθυνση Βιολογία Στόματος (Βασικές Οδοντιατρικές Επιστήμες)
Βιβλιοθήκη Οδοντιατρικής
Deposit date:
2019-07-16
Year:
2019
Author:
Doukaki Dafni
Supervisors info:
Ευθυμία Κιτράκη, Καθηγήτρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας,ΕΚΠΑ
Νικόλαος Κερεζούδης ,Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας,ΕΚΠΑ
Χρήστος Ραχιώτης , Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας,ΕΚΠΑ
Original Title:
Ανοσοϊστοχημική μελέτη πρώιμων αποκρίσεων του πολφού έπειτα από άμεση κάλυψη, σε ένα ex vivo μοντέλο ανθρώπινου δοντιού
Languages:
Greek
Translated title:
Immunohistochemical study of early dental pulp responses after direct pulp capping in an ex vivo human tooth model
Summary:
Aim: The aim of this study was to investigate tissue integrity and cell stress levels, in human dental pulp, immediately after placement of adhesive composite resin systems (Prime&Bond, Clearfil SE και Clearfil S3) and calcium hydroxide paste (Dycal) on it.

Materials and Methods: The study was conducted in cryotome sections of human tooth pulp. These sections derived from tooth slices that were cultured for four days upon the application of the agents under study. We performed immunohistochemistry in cryotome sections of these samples in order to identify the localization and expression levels of the following proteins: osteopontin (OPN), dental sialoprotein (DSP), stress protein of the endoplasmic reticulum (Endoplasmic reticulum - (ER) resident protein containing DNA J, DNAJ10 / ERdj5) and thioredoxin reductase 1 (TRXR1). The pulp areas close and at a distance from the application point were examined under the optical microscope and digitized images were used for quantification of the immunohistochemical signal through appropriate software. The statistical evaluation of the results was done by one way analysis of variable.

Results: The results showed that each agent had different effect in pulp cells. The cellular responses to the applied agents varied, not only compared to the control group, but among the experimental groups as well. Regarding OPN, statistically significant effects were observed both in the areas of the dentinal layer of the tooth crown and around it, as well as in the whole pulp. Specifically, OPN levels in the whole pulp were increased in all experimental groups relative to the control group. The higher increase was detected in the Clearfil SE group. A statistically significant increase was also seen in the area of the dentinal layer for the Prime & Bond, Clearfil SE and Dycal groups relative to the control group. Statistically significant changes were observed in whole pulp for the cellular stress marker proteins ERdj5 and TRXR1. ERdj5 levels were reduced in the Clearfil S3 group compared to the control group, while TRXR1 levels were increased in the Prime & Bond group relative to the control group. DSP levels did not change significantly among groups.

Conclusions: The results of the present study, indicate the need for further research of the complex mechanisms of cellular responses, as well as the possibly negative effects of pulp capping materials.
Main subject category:
Health Sciences
Keywords:
Cellular responses , Dental pulp , Proteins , Capping materials
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
117
Number of pages:
93
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