Unit:
Specialty NanomedicineLibrary of the School of Health Sciences
Author:
Zouliati Konstantina
Supervisors info:
Κωνσταντίνος Δεμέτζος, Καθηγητής, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Μαριλένα Βλάχου, Επίκουρη Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Άρης Ξενάκης, Διευθυντής Ερευνών, Ινστιτούτο Χημικής Βιολογίας, Εθνικό Ίδρυμα Ερευνών
Original Title:
Design, development and evaluation of liposomal nanoparticles. In vitro toxicity and permeability study with Transwell methodology.
Translated title:
Design, development and evaluation of liposomal nanoparticles. In vitro toxicity and permeability study with Transwell methodology.
Summary:
The aim of this study was to prepare liposomal systems, examine their physicochemical characteristics and thermal behavior overtime and evaluate their toxicity and permeability properties in vitro using a co-culture of intestinal epithelial cells Caco-2/TC7 and HT29-MTX. Liposomal systems were prepared using different lipids and physicochemical characterization was performed over a period of 31 days, showing satisfactory results in terms of physicochemical stability. Differential scanning calorimetry was performed to evaluate the thermal behavior of liposomes, displaying the role of each lipid in the formation of the thermodynamic characteristics of each system. Their toxicity was evaluated using MTT assay and all liposomes presented high viability of cells. Particularly, none of the systems caused viability lower than 80% even at the highest concentrations used (15% v/v). Therefore, the systems were non-toxic to the epithelial cells and permeability studies were performed using rhodamine B as a tracer dye. Permeability studies showed 2-2.5 folds permeability enhancement by the use of liposomes compared to the solution of rhodamine B. Among these systems, the liposomes containing the positively charged lipid DOTAP presented the highest percentage of transport of rhodamine B across the epithelial monolayer. Liposomes can be promising drug delivery systems across the intestinal epithelium, however further research needs to be done to examine the exact mechanisms of liposomal absorption and overcome in vivo barriers that eliminate the efficiency of liposomes.
Main subject category:
Health Sciences
Keywords:
Nanomedicine, Liposomes, Intestinal Permeability, MTT
